Effects of manganese fertilizer on wheat leaf senescence under different water conditions
Drought is one of the main factors restricting wheat productivity and it is important to explore the effects of manganese on wheat leaf senescence under different soil water conditions.In an outdoor pot experiment,three levels of soil water treatments(CK:normal water,which was 70％-75％of the maximum field water capacity,MS:moderate water shortage,which was 50％-55％of the maximum field water capacity,SS:severe water shortage,which was 40％-45％of the maximum field water capacity)and five concentrations of MnSO4 solution(0％,0.2％,0.5％,1.0％,2.0％)spraying on wheat leaves during booting and flowering period were set up.The activities of superoxide dismutase(SOD),peroxidase(POD)and malondialdehyde(MDA)in wheat leaves were determined.The fitting results showed that the lowest MDA content in wheat leaves after two spraying treatments was 3.19 and 4.04 g/L under CK treatment respectively,which was still lower under MS and SS treatments and the MDA content in wheat leaves was significantly reduced.When POD activity reached its maximum,the corresponding manganese concentrations were 5.13 and 4.30 g/L under MS treatment respectively,and the corresponding manganese concentrations were significantly different under the CK and SS treatment.When SOD activity reached its maximum after two sprays,the corresponding manganese concentration was above 10 g/L and SOD activity was increased under each treatment.The application of manganese fertilizer reduced the MDA content of wheat leaves and increased the activities of SOD and POD,which could delay the senescence of wheat leaves under different soil water conditions,but the optimal concentration was different.
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