Isolation and screening of an anthracene-degrading strain and preliminary analysis of its degradation characteristics
Polycyclic aromatic hydrocarbons(PAHs)are the most common typical persistent organic pollutants in soil.Anthracene,considered as the typical representative,is often used as a model compound for degrading PAHs and an indicator for detecting PAHs contamination.The contaminated soil samples were collected from the suburbs of Beijing,and 185 strains with anthracene degradation potential were isolated after domestication and enrichment.Moreover,the anthracene degradation efficiency was quantified by high performance liquid chromatography(HPLC),and a strain R4004-2 with high anthracene degradation efficiency was obtained.According to colonial morphology,phylogenetic analysis of 16S rDNA and gyrB,as well as ANI,DDH,dDDH of genomics,the strain R4004-2 was identified as Pseudomonas stutzeri.Pseudomonas stutzeri R4004-2 grew well when anthracene was used as the sole carbon source.It could degrade up to 82.3％of anthracene and grew fastest in broth medium.In contaminated soil with anthracene content of 0.25 mg/kg,Pseudomonas stutzeri R4004-2 could effectively colonize,indicating the potential to remediate contaminated soil.Based on the transcriptomic and metabolomic results,Pseudomonas stutzeri R4004-2 was able to oxidize anthracene in the first step through dioxygenase,and then complete the degradation of anthracene through the oxidation of anthraquinone and the decomposition of 1-hydroxy-2-naphthoate,respectively,and initially resolved its degradation mechanism.
万方数据
维普
