Isolation,identification and degradation characteristics of maize straw of low-temperature cellulose-degrading strain Pseudogymnoascus sp.SDF-LT
In order to screen low-temperature and high-efficiency cellulose-degrading fungi,39 soil samples were collected from the wheat-maize double cropping fields in Shijiazhang,Xingtai,Tangshan in Hebei Province.The size of the transparent circle of the straw degradation fungi were screened by Cellulose sodium-Congo red medium at 10℃.By comparing the filter paper enzyme activities,a low temperature cellulose degrading fungi SDF-LT was found.Based on the morphology and ITS phylogenetic analysis,the fungi were identified as a strain of the genus Pseudogymnoascus.Sandbag method under natural low-temperature conditions was used to determine the straw degradation ability of the strains SDF-LT.The degradation rate of this strain was 42.67%at 80 days after inoculation,which was significantly higher than that of the normal temperature inoculants and control.At 80 days,the content of cellulose,hemicellulose,and lignin in maize straw treated with SDF-LT were 26.79%,28.52%,4.41%respectively,which were significantly lower than those of normal temperature straw degrading bacteria and control.Using scanning electron microscopy,it was found that the original fiber bundle structure of straw treated by SDF-LT was broken,and the fiber structure was highly damaged.The screened strain SDF-LT provided strain resources for the maize straw degradation.
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