高效液相色谱法同时测定肥料中两种抑制剂的含量及国际标准制定研究
Simultaneous determination of two inhibitors in fertilizers by high performance liquid chromatography and development of related international standard
王新慧 1田烨炜 1黄河清 1颜坤 1储德韧2
作者信息
- 1. 上海化工研究院有限公司,上海 200062;上海化工院检测有限公司,上海 200062
- 2. 上海化工研究院有限公司,上海 200062
- 折叠
摘要
为解决氮肥增效助剂测定方法存在的问题并实现对新型肥料的有效管控,建立了对肥料中N-正丁基硫代磷酰三胺和双氰胺同时测定的高效液相色谱分析方法.检验结果表明,该方法在线性范围内2 种抑制剂的浓度与对应色谱峰面积的线性关系良好,线性相关系数均＞0.9999,回收率分别为91.1%～104.8%和90.9%～107.6%,6 次平行测定的相对标准偏差分别为 1.94%和 0.84%.与此同时,通过对全球 18 家国际实验室间比对试验数据的统计分析,表明该方法在兼顾前处理简单、分离效果好、检测准确的同时,又具有适用性广、精密度高的优势,ISO/DIS 6650 可作为新的国际标准推广使用.
Abstract
In order to solve the problem existing in the determination method of nitrification inhibitors and achieve effective management and control of new fertilizers,an analytical method for simultaneous determination of NBPT and DCD in fertilizers was established by high performance liquid chromatography.The results showed that the linear relationship between the mass concentration of the two inhibitors and the value of the corresponding chromatographic peak area was good in the linear range.The correlation coefficients were all greater than 0.9999.The recoveries were 91.1%-104.8%and 90.9%-107.6%,and the relative standard deviations of 6 parallel determinations were 1.94%and 0.84%,respectively.At the same time,through the statistical analysis of an international comparison test between 18 international laboratories world-widely,it indicated that the method not only had the advantages of simple pretreatment,good separation effect and accurate detection,but also had the advantages of wide applicability and high precision.New international standard of ISO/DIS 6650 was a good testing method which could be used world-widely.
关键词
高效液相色谱/N-正丁基硫代磷酰三胺/双氰胺/国际标准Key words
high performance liquid chromatography/NBPT/DCD/international standard引用本文复制引用
出版年
2024
NETL
NSTL
万方数据