The effect of nitrogen application on the soil fungal community of Korla fragrant pear orchard
The aimed to explore the effects of long-term different nitrogen levels on the structural composition and diversity of soil fungal communities(0-20 cm soil layer)in Korla fragrant pear(Pyrus brestschneideri Rehd.)orchards,and to provide a theoretical basis for the rational application of nitrogen fertilizer in the production process of Korla fragrant pear.Based on the long-term fertilization experiment,the relationships between soil physicochemical properties and soil fungal community composition in Korla fragrant pear orchard under four nitrogen application rates of N 0,150,300 and 450 kg/hm2(expressed as N0,N1,N2 and N3,respectively)were studied by using high-throughput sequencing technology.The results showed that Ascomycota,Basidiomycetes and Mortierella were the dominant fungal groups in the soil of all fertilization treatments.The dominant fungal genera were Aspergillus,Acrosporase,Alternaria,Staphylococcus,and Pseudomonas.Carbon and nitrogen contents and soil physicochemical properties significantly affected the composition of microbial communities,while high nitrogen fertilization increased the richness of fungi.Medium to high nitrogen fertilization enhanced fungal diversity and community distribution uniformity,and soil fungal community biodiversity was mainly affected by carbon and nitrogen components contents,electrical conductivity,and pH.
Korla fragrant pearnitrogen fertilizermicrobial diversityfungiphysical and chemical properties of soil