Effects of adding polyaspartic acid on soil nitrogen leaching and nitrogen utilization of tobacco plants
In order to investigate the effects of adding polyaspartic acid(PASP)on soil nitrogen leaching loss and nitrogen utilization of tobacco plants,soil column leaching test with different PASP addition ratio and pot experiment with PASP addition under different nitrogen fertilizer gradients were set up to study the effects of PASP on soil mineral nitrogen leaching,tobacco plant growth,chlorophyll content,photosynthetic rate,key enzyme activity of carbon and nitrogen metabolism,chemical composition and nitrogen utilization rate.The results showed that adding PASP reduced the leaching loss of nitrate nitrogen and ammonium nitrogen in soil,and the greater the proportion of PASP added,the better the leaching loss reduction effect.Compared with the control group,under conventional nitrogen application and 10%nitrogen reduction conditions,adding PASP improved the growth of tobacco plants,increased the net photosynthetic rate,enhanced the activity of key enzymes in carbon and nitrogen metabolism and root activity,increased dry matter and nitrogen accumulation,and increased the nitrogen utilization rate by 57.74%and 42.54%,respectively.The tobacco plant growth of PASP treated with 20%nitrogen reduction was slightly weaker,except sucrose synthase(synthesis direction)activity,other physiological indexes were slightly decreased on the whole,total nitrogen and sugar contents were decreased,nitrogen accumulation was decreased,and nitrogen utilization rate was similar to that of the control group.It was concluded that the application of 5%PASP under the condition of 10%nitrogen reduction could reduce the soil nitrogen leaching loss and increase the nitrogen utilization rate of tobacco plants.
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