Effects of leaf spraying oligosaccharides on the community structure of azotobacter in the rhizosphere and the yield of peanut
Field experiment was conducted to study the effects of chitosan oligosaccharides and cello-oligosaccharide on the community structure of azotobacter in the rhizosphere and the yield of peanut.The experiment included three treatments on the surface of leaf:clean water(CK),chitosan oligosaccharides 50 mg/L(CSOS)and cello-oligosaccharide 50 mg/L(COS).High throughput sequencing technology was used to analyze the community structure and diversity of azotobacter in the rhizosphere.(1)Compared to CK,the relative abundance of Bradyrhizobium,Vitreoscilla,Azohydromonas,Azospirillum,Pseudomonas,Calothrix and Frankia,significantly increased of CSOS treatment by 10.99%,45.63%,29.88%,23.24%,181.13%,27.75%and 36.61%,respectively.The relative abundance of Azospirillum,Sinorhizobium and Calothrix of COS treatment significantly increased by 86.12%,664.41%and 277.97%,respectively.(2)Compared with CK,the CSOS treatment significantly increased the content of total nitrogen and ammonium nitrogen in the soil with an average increase of 15.50%and 20.19%,respectively.COS treatment significantly increased total nitrogen,nitrate nitrogen and ammonium nitrogen in the soil by 17.05%,18.68%and 27.58%,respectively;In addition,soil pH decreased significantly by 0.08 and 0.06 for both CSOS and COS treatments compared to CK,respectively.(3)Both CSOS and COS treatments significantly increased peanut yield by 19.3%and 22.0%,respectively.Therefore,foliar sprays of chitosan oligosaccharides and cellooligosaccharide regulated the relative abundance of different nitrogen-fixing bacterial genera and affected soil physicochemical factors(total nitrogen,nitrate nitrogen and ammonium nitrogen)in the root zone of peanut,all of which ultimately significantly increased peanut yield.
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