Field experiment was conducted to study the effects of chitosan oligosaccharides and cello-oligosaccharide on the community structure of azotobacter in the rhizosphere and the yield of peanut.The experiment included three treatments on the surface of leaf:clean water(CK),chitosan oligosaccharides 50 mg/L(CSOS)and cello-oligosaccharide 50 mg/L(COS).High throughput sequencing technology was used to analyze the community structure and diversity of azotobacter in the rhizosphere.(1)Compared to CK,the relative abundance of Bradyrhizobium,Vitreoscilla,Azohydromonas,Azospirillum,Pseudomonas,Calothrix and Frankia,significantly increased of CSOS treatment by 10.99%,45.63%,29.88%,23.24%,181.13%,27.75%and 36.61%,respectively.The relative abundance of Azospirillum,Sinorhizobium and Calothrix of COS treatment significantly increased by 86.12%,664.41%and 277.97%,respectively.(2)Compared with CK,the CSOS treatment significantly increased the content of total nitrogen and ammonium nitrogen in the soil with an average increase of 15.50%and 20.19%,respectively.COS treatment significantly increased total nitrogen,nitrate nitrogen and ammonium nitrogen in the soil by 17.05%,18.68%and 27.58%,respectively;In addition,soil pH decreased significantly by 0.08 and 0.06 for both CSOS and COS treatments compared to CK,respectively.(3)Both CSOS and COS treatments significantly increased peanut yield by 19.3%and 22.0%,respectively.Therefore,foliar sprays of chitosan oligosaccharides and cellooligosaccharide regulated the relative abundance of different nitrogen-fixing bacterial genera and affected soil physicochemical factors(total nitrogen,nitrate nitrogen and ammonium nitrogen)in the root zone of peanut,all of which ultimately significantly increased peanut yield.
NETL
NSTL
万方数据
