Effects of biochar-based fertilizer on the physiological characteristics of stress resistance and economic traits of flue-cured tobacco
In order to explore the effects of biochar-based fertilizer on antioxidant enzyme,osmotic regulation ability and economic traits of flue-cured tobacco,'Yunyan 116'was chosen as the research object and three treatments were set up,including conventional fertilization(T1),biochar-based organic fertilizer(T2)and biochar-based compound fertilizer(T3).The physiological characteristics of stress resistance of leaves and roots were determined at 30,60,90 and 120 d after transplanting,including activities of superoxide dismutase(SOD)and peroxidase(POD)and contents of malondialdehyde(MDA),soluble protein(SP),soluble sugar(SS)and proline.Additionally,the yield,output value,average price,and ratio of upper middle grade leaves of tobacco after curing were analyzed.The main results were as follows.(1)Biochar-based fertilizer improved the stress resistance and osmotic adjustment ability of tobacco plants,and T2 treatment had the best effect.The activities of SOD and POD in leaves increased by 1.1%-10.6%and 2.1%-9.5%,respectively,compared with T1 treatment,and the content of MDA decreased by 16.0%-20.5%.At the same time,the contents of SP,SS,and proline in leaves treated with T2 and T3 increased by 1.4%to 102.1%,16.0%to 52.0%,and 7.8%to 41.1%,respectively.The root stress resistance characteristics of different treatments were consistent with those of leaves.(2)Biochar-based fertilizer improved the yield,output value and ratio of upper middle grade leaves of flue-cured tobacco,with the highest yield and output value in T2 treatment,which increased by 16.9%and 21.8%compared to T1 treatment,respectively.(3)Through the correlation analysis of physiological and economic traits of flue-cured tobacco,it could be seen that there were varying degrees of correlation between the two types of indicators during the four measurement periods,with the overall correlation being the closest at 120 d after transplantation.In summary,the application of biochar-based fertilizer improved the antioxidant capacity,osmotic regulation ability,and economic traits of flue-cured tobacco,among which T2 treatment had the best effect.The research results could provide theoretical basis and technical support for the rational application of biochar-based fertilizer.
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