Effects of the rice-stropharia grandis rotation/cultivation on soil microbial biomass C,N and P
In order to investigate the effects of the cultivation of rice-stropharia grandis and its strain dosage and base material formula on soil nutrients and soil microbial biomass C,N and P(MBC,MBN,MBP),a 2-year field plot experiment was carried out and 5 treatments were set up,including control treatment(CK)and four experimental treatments:T1(3000 kg·hm-2 strain+100%rice straw base material),T2(3000 kg·hm-2 train+75%rice straw with 25%corncob base material),T3(4500 kg·hm-2 strain+100%rice straw base material)and T4(4500 kg·hm-2 strain+75%rice straw with 25%corncob base material).The results showed that the content of soil nutrients and soil microbial biomass C,N and P in the cultivated layer of rice-stropharia grandis for two consecutive years increased significantly year by year,and was affected by the strain dosage and base material formula.In the second year,compared with the CK treatment,the soil organic matter,alkaline nitrogen,available phosphorus,available potassium,total nitrogen and total phosphorus of each experimental treatment increased significantly by 32.92%-42.44%,52.96%-77.35%,53.99%-75.09%,88.00%-184.62%,19.12%-38.24%,9.82%-29.46%(P<0.05),respectively,soil MBC and MBN content increased significantly by 84.18%-129.28%,166.60%-260.01%(P<0.05),respectively.During the 2 years,the ratio of MBC:MBN decreased significantly,while the ratio of MBC:MBP and MBN:MBP increased in each experimental treatment,compared with CK treatment.Redundancy analysis(RDA)showed that total nitrogen explained 31.1%and 36.3%of soil microbial biomass changes in 2021(F=5.9,P=0.004)and 2022(F=7.4,P=0.002),respectively,which were the most important influencing factors of soil MBC,MBN,MBP and its stoichiometric ratio.Correlation analysis showed that the contents of soil MBC and MBN were significantly positively correlated with the contents of organic matter,total nitrogen,total phosphorus,alkaline nitrogen,available phosphorus and available potassium.The cultivation of rice-stropharia grandis not only significantly increased soil nutrients,but also increased soil MBC,MBN,MBP contents,T2(3000 kg·hm-2 strain+75%rice straw with 25%corncob base material)was the best cultivation method.This study can provide theoretical guidance for the subsequent popularization of rice-stropharia grandis rotation model.