Analysis of dynamic changes of rhizosphere bacterial communities in the pathogenesis of tobacco bacterial wilt
In order to understand the dynamic changes of soil microbial community during the whole disease process of tobacco bacterial wilt in cultivation areas of tobacco of Shandong Province,this study systematically analyzed the tobacco rhizosphere microbial community in Yishui County,Shandong Province by high-throughput sequencing.Amplicon sequencing analysis showed that,compared with before transplanting,the microbial diversity of rhizosphere soil significantly decreased when tobacco bacterial wilt was initially manifested,but there was a trend of recovery in the subsequent rhizosphere soil microbial diversity with the development of the disease.The microbial composition in tobacco rhizosphere before transplanting and early onset of tobacco bacterial wilt were compared,and the rule between the dynamic process of the whole disease occurrence and the change of rhizosphere bacterial community was explored.Via a constructed correlation network,Granulicella,Achromobacter,Gemmatimonas,Humibacter,Flexivirga and Conexibacter were detected to have potential effect on the occurrence of bacterial wilt.In summary,the difference of rhizosphere bacteria composition in a time-dimensional process of tobacco onset was analyzed,and the potential bacteria genera with disease control and warning potentials were found,which laid a foundation for the early warning and control of soil-borne tobacco diseases in Shandong.