Biochar as a soil amendment could effectively improve soil pollution problems.Exploring the impacts of biochar and nitrogen fertilizer combination on soil bacterial community were important for the scientific fertilization.Previous studies have found that the combination of biochar and nitrogen fertilizer can alter soil physicochemical properties,but there is little research on its impact on soil microorganisms.To investigate the effect of biochar and nitrogen fertilizer combination on soil bacterial community,a pot experiment was conducted on rice and the results showed that the diversity of bacterial communities decreased with the application of biochar and nitrogen fertilizer.In the improved soil,Proteobateria,Acidobaceria,Gemmatimonadetes,and Actinobacia were the most prevalent phyla of bacteria.The use of biochar and nitrogen fertilizer enhanced the relative abundances of Acidobacteria and Actinobacteria while decreased the relative abundances of Proteobacteria and Gemmatimonadetes.In addition,redundancy analysis showed that soil pH value,nitrate nitrogen,total phosphorus and total nitrogen were the main driving factors that affected the species richness of soil microbial community markers.The combination of biochar and nitrogen fertilizer could alter the metabolic process of the soil bacterial community and soil nutrient content,which was related to soil bacterial community marker species.This study provided scientific basis for exploring the best carbon nitrogen model suitable for the regulation of soil microenvironment in the black soil area of Northeast China.
关键词
生物炭/氮肥/16SrDNA测序/微生物群落结构/土壤养分指标
Key words
biochar/nitrogenous fertilizer/16S rDNA sequencing/microbial community structure/soil nutrient index
维普
万方数据