Effects of long-term application of organic fertilizer on soil bacterial community characteristics of facility vegetables
The aimed to explore the impact of long-term application of organic fertilizers on the structural characteristics of soil bacterial communities in greenhouse vegetable cultivation.A 13-year field experiment with greenhouse vegetables was conducted,comprising four treatments:no nitrogen fertilezer and organic fertilizer application(D0),sole application of organic fertilizer(D1),combined application of organic and inorganic fertilizers(D2),and nitrogen-enriched combined organic and inorganic fertilizers(D3).Using metagenomic sequencing technology,the differences in soil bacterial community structures among these treatments were compared.Long-term application of organic fertilizers significantly increased the content of total nitrogen,total phosphorus,total potassium,alkali-hydrolyzable nitrogen,available phosphorus,available potassium and organic matter in greenhouse vegetable soil(P<0.05).It also increased the relative abundance of Actinobacteria and decreased that of Proteobacteria.Sole organic fertilizer application significantly increased the relative abundance of Nitrospirae(P<0.05),while combined organic and inorganic fertilizer application significantly increased the relative abundance of Verrucomicrobia and Planctomycetes(P<0.05).Redundancy analysis indicated that soil environment explained 83.66%of the variation in bacterial community structure.Correlation analysis revealed that soil pH,total phosphorus,total potassium and alkali-hydrolyzable nitrogen contents were the main environmental factors influencing bacterial community structure.Planctomycetes were significantly negatively correlated with pH(P<0.05)and positively correlated with nutrient content.Chloroflexi showed a negative correlation with nutrient content,especially with total phosphorus,total potassium and alkali-hydrolyzable nitrogen contents(P<0.05).Firmicutes were significantly negatively correlated with total potassium content(P<0.05).The results of this study indicated that long-term application of organic fertilizers significantly increased nutrient content in greenhouse vegetable soil,effectively enhanced the relative abundance of soil bacteria,and improved soil bacterial community structure.
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