基于微生物组学分析揭示土壤调理剂促进水稻增产的机制
Elucidation of the mechanisms of application of soil conditioning agents to promote rice grain yield based on microbiomics analysis
余垚颖 1黄小琴 1杨潇湘 1邓越 1张重梅 1周西全 1向运佳 1鲜赟曦 1刘勇 1张蕾1
作者信息
- 1. 四川省农业科学院植物保护研究所,四川 成都 610066;农业农村部西南作物有害生物综合治理重点实验室,四川 成都 610066
- 折叠
摘要
为了解析土壤调理剂促进水稻增产的机制,开展施用土壤调理剂(TS)和未施用土壤调理剂(CS)的大田试验,通过分析土壤养分指标探究调理剂对土壤理化性质的改善效果,借助高通量测序技术测定施用调理剂对土壤微生物群落结构的影响.结果表明:施用专用调理剂(碱性)后,(1)水稻单产增加 810.15 kg·hm-2(6.83%);(2)土壤养分状况得到明显改善,土壤pH和交换性钙离子含量显著提高;(3)地杆菌科、噬几丁质科、亚硝化单胞菌科、Nitrososphaeraceae、假球壳科和Ustilaginaceae的相对丰度明显提高;(4)相对丰度前30 的科中,土壤pH与噬几丁质科、黄色杆菌科、Bacteroidetes_vadinHA17、嘉利翁氏菌科、葫芦腔菌科呈显著负相关,与Nitrososphaeraceae和Ustilaginaceae呈显著正相关;土壤交换性钙离子与黄色杆菌科、Bacteroidetes_vadinHA17 和地根壶菌科呈显著负相关,与Nitrososphaeraceae和Ustilaginaceae呈显著正相关.综上所述,施用专用土壤调理剂(碱性)能改善土壤理化性质和微生物群落结构,从而有利于水稻干物质积累和产量提升.
Abstract
In order to analyze the mechanisms for promoting rice yield by the soil-conditioning agents(SCAs),a field experiment was carried out,and treatments with SCAs(TS)and without SCAs(CS)were conducted.The improvement effect of SCAs on soil physicochemical properties was explored by analyzing soil nutrient,and high-throughput sequencing technology was used to determine the impact of application of SCAs on soil microbial community structure.The results showed that,after applying the SCAs(alkaline),(1)the rice yield increased by 810.15 k·hm-2(6.83%);(2)The soil nutrient status was significantly improved,with a significantly increasing soil pH value and exchangeable calcium ion content;(3)The relative abundance of Geobacteraceae,Chitinophagaceae,Nitrosomonadaceae,Nitrososphaeraceae,Pleosporaceae and Ustilaginaceae was significantly increased;(4)Among the top 30 families with relative abundance,soil pH was significantly negatively correlated with the relative abundance of Chitinophagaceae,Xanthobacteraceae,Bacteroidetes_vadinHA17,Gallionellaceae and Cucurbitariaceae,and significantly positively correlated with Nitrososphaeraceae and Ustilaginaceae;The content of exchangeable calcium ions in soil was negatively correlated with the relative abundance of Xanthobacteraceae,Bacteroidetes_vadinHA17 and Terramycetaceae,and significantly positively correlated with Nitrososphaeraceae and Ustilaginaceae.These results suggested that application of the SCAs(alkaline)could improve soil physicochemical properties and alter soil microbial structure,thereby leading to the enhancing accumulation of biomass and increasing of yield of rice.
关键词
水稻/增产/土壤调理剂/高通量测序/微生物群落结构Key words
rice/production increase/soil-conditioning agents/high-throughput sequencing/microbial community structure引用本文复制引用
出版年
2024
NETL
NSTL
万方数据