Phosphate solubilization bacteria screening from urban green space soil and their growth promoting effect analysis
The phosphate-solubilizing bacteria from the soil environment of urban green space were screened and their genera were identified,in order to obtain dominant phosphate-solubilizing strains with growth-promoting effects.Phosphate-solubilizing bacteria from screening plates were obtained by observing the phosphate-solubilizing halo,and the phylogenetic tree was constructed by analysing the homology of 16S rDNA fragments.The phosphate solubilization ability of the strains was quantified with molybdenum antimony anti-coloring method,and pH of the medium was also measured.Selecting three strains with strong phosphate solubilization ability,corresponding to T1,T2 and T3 treatments,to test their growth promoting effect through Petunia hybrida cultivation experiment,non-inoculated strain treatment(CK)was set as control.The result showed that 29 phosphate-solubilizing bacteria were screened from the green space soil,and they belonged to Kluyvera,Pantoea,Enterobacter,Burkholderia,Paraburkholderia and Ochrobactrum genera.The phosphate solubilization ability of the strains ranged from 8.67 to 632.62 mg/L,and the corresponding pH distribution of the media ranged from 6.38 to 4.42.There was a significant negative correlation between them(R2=0.8775).The three strains significantly increased the crown width,leaf number,flower bud number,and dry weight of P.hybrida,but had no significant effect on plant height and SPAD.T3 strain significantly increased root volume,and T2 and T3 strains both increased phosphorus content in the aboveground and underground parts of the plant,while T1 strain had no significant promoting effect on the above growth indicators.Overall,the ability of the 29 phosphate-solubilizing bacteria to acidify the culture media could reflect their phosphate solubilizing ability to a certain extent.The three selected strains all had growth promoting effects,and the T3 strain with the strongest phosphate solubilization ability performed the best and possessed the potential for further development as a microbial fertilizer.
urban green space soilphosphate-solubilizing bacteriaPetunia hybridgrowth promoting
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