Allelopathic effect of cotton in seedling stage on sunflower broomrape
The effect of extracts from five cotton (Gossypium hirsutum L.) varieties (Zhongmiansuo 63, Zhongmiansuo 57,Zhongmiansuo 47, Zhongmiansuo 44, and Zhongmiansuo 41) on sunflower broomrape (Orobanche cumana Wallr.) germination were tested. The cotton varieties were grown in separate pots. When the plants were at the two, four, and six-leaf stages, samples were collected from the rhizosphere soil as well as from the roots, stems, and leaves of the cotton plants. The samples were extracted with methanol and then the extracts were applied to sunflower broomrape seeds. The results indicated that seeds treated with rhizosphere extracts germinated first, followed by those treated with root extracts, stem extracts, and finally leaf extracts.Germination rates were highest when the seeds were treated with rhizosphere or plant extracts collected at the two-leaf stage. The varieties of cotton had no significant effect on sunflower broomrape germination when the seeds were treated with extracts collected at the two-leaf stage. In contrast, there were significant differences among the varieties when the seeds were treated with extracts collected at the four and six-leaf stages. In conclusion, cotton plants at the two-leaf stage had the strongest allelopathic effect on sunflower broomrape. These results provide a basis for using cotton as a “trap crop” for the bio-control of the parasitic weeds.