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Cr6+对3种玉米品种的生理特性影响

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为了研究不同剂量下Cr6+对于三种玉米(Zea mays L.)种子的毒害效应,进一步探究长期堆放铬渣的土壤对周围环境的污染程度以及对生态环境的潜在影响。选取三种不同品种的玉米,随机分为对照组以及1×10-4、5×10-4、1×10-3 mol·L-1,5×10-3、1×10-2 mol·L-1实验剂量组,探究Cr6+对三种不同品种玉米种子的毒害效应。将种子于实验室条件下常规培养后,分别测定不同Cr6+胁迫剂量下,三种玉米种子(郑单958,强盛31,农大108)的生长状况,株高、根长、鲜物质量和干物质量以及胁迫前后超氧化物歧化酶(SOD)、过氧化物酶(POD)以及丙二醛(MDA)的含量变化。对不同剂量下Cr6+对三种玉米的生理特性影响进行比较研究。结果表明:5种不同浓度的Cr6+溶液对玉米种子进行胁迫后,三种玉米的生长均受到了一定程度的抑制,玉米幼苗株高、根长、鲜物质量和干物质量均呈下降趋势。在低剂量1×10-4 mol·L-1时,即产生显著影响(P<0.05),且胁迫剂量越大,对玉米种子生长产生的抑制作用越强。三种不同的玉米种子体内均产生了Cr6+富集现象,且随着染毒剂量的增加,玉米体内富集量增加,细胞叶绿素含量和叶绿素a/b比值降低,三种玉米品种幼苗POD活性、SOD活性以及MDA含量均随暴露浓度增加而增加。在Cr6+暴露剂量为最大剂量1×10-2 mol·L-1时,POD活性、SOD活性与MDA含量均增加至最大值。各剂量下,均有显著性变化(P<0.05)产生。由此可见,不同剂量的Cr6+对于三种玉米种子均会产生一定的毒害效应,对玉米的生长产生不良影响。
Effects of Cr6+on Physiological Characteristics of Three Species of Corns
To study the toxicity effect of Cr and the long-term chromic slag dumps' impact on the surrounding ecological environment and the present status for chromic slag pollution. Three different kinds of maize seeds (Zhengdan 958, Qiangsheng 31, Nongda 108) were divided into the controls, 1×10-4, 5×10-4, 1×10-3 mol·L-1, 5×10-3, 1×10-2 mol·L-1 Cr6+ to determine the toxicity effect to the maize. The seeds were routine cultured in the laboratory, then the plant height, root height, plant dry weight, wet weight, the chlorophyll content and the changes of the activity of superoxide dismutase (SOD), peroxidase (POD) and malondialdehyde (MDA) were measured. The results shown that compared with the controls, the plant height, root height, plant dry weight and wet weight decreased respectively in dose-effect manner following the rising Cr6+. The change tendency showed significant differences (P<0.05) with 1×10-4 mol·L-1 Cr6+. As the content of Cr6+ increased, the chlorophyll content was decerased and the activities of dismutase (SOD), peroxidase (POD) and malondialdehyde (MDA) were gradually increased. The statistically significant differences (P<0.05) were observed at each dose of experimental group. The Cr6+could cause toxicity effect of maize seeds and do harm for the growth of the maize.

Cr6+CornSODMDAPOD

马溪平、李鲜珠、沈玉冰、李万龙、张宏亮、王迪、徐成斌

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辽宁大学环境学院,辽宁 沈阳 110036

Cr6+ 玉米 SOD MDA POD

2011年辽宁省科学技术计划项目辽宁大学环境学院重点学科项目

2011230009

2014

生态环境学报
广东省生态环境与土壤研究所 广东省土壤学会

生态环境学报

CSTPCDCSCD北大核心
影响因子:1.608
ISSN:1674-5906
年,卷(期):2014.(9)
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