摘要
目的：从废弃羽毛堆积处土壤中分离筛选具有高效降解羽毛角蛋白的细菌。方法形态学观察、生化测定和16 SrDNA 序列分析鉴定菌株，测定蛋白水解研究酶活性检及酶特性。结果鉴定该菌为嗜麦芽窄食单胞菌，降解羽毛的最适发酵培养温度为30℃，最适 pH 是7.0，Fe2+对酶活有促进作用，Cu2+、Zn2+、Mn2+对酶活有明显抑制作用。结论嗜麦芽窄食单胞菌角蛋白酶的作用温度和 pH 宽泛、稳定性好，具有良好的开发价值和应用前景。
Abstract
Objective:To isolate and characterize feather-degrading bacteria from soil of discarded feathers. Methods:Morphological observation,biochemical analysis and 16SrDNA sequence analysis were used for identifying bacteria,and the enzyme activity and enzyme properties were studied by mensurating protein hydrolysis. Results:The strain was identified as a strain of the genus Pseudomonas,The optimal pH and temperature for growth were 7. 0 and 30 °C,Fe2 + promote the ac-tivity of the enzyme,Cu2 + ,Zn2 + ,Mn2 + inhibited the activity of the enzyme. Conclusion:This bacteria has the potential for the application of feather keratin sources because the keratinase of Stenotrophomonas maltophilia functional temperature and pH are broad and stable.
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维普
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