摘要
目的：探讨嗅鞘细胞（OECs）静脉移植治疗陈旧性脊髓损伤的可行性以及疗效。方法制备 wistar 大鼠T10左侧脊髓半切损伤模型（不做任何处理，自行恢复1个月），大鼠的神经功能恢复变缓慢，进入平台期，并通过CBS 评分法，大鼠的评分在47分左右，随机分成3组（每组10只）：OECs 静脉移植组（A 组）、D/ F12细胞培养液静脉移植组（B 组）、空白对照组（C 组）。通过 CBS 评分法定期对大鼠的神经功能进行评价，通过组织形态学观察脊髓损伤部位组织的修复情况。结果 CBS 评分显示：从第2周开始在每个时间段 A 组与 B 组、C 组均有显著差异（P ＜0.05），B、C 组之间没有显著性差异（P ＞0.05）。镜下观察可见：A 组与 B 组、C 组有明显差别，A 组瘢痕组织小于 B 组和 C 组，可见神经纤维轴突再生，脊髓修复明显优于 B 组、C 组，B、C 组无明显差别。结论 OECs 静脉移植对陈旧性脊髓损伤修复有一定的疗效。此种移植方式既操作简单，又具有较好的疗效，并且可以重复多次进行，具有良好的临床推广和应用价值。
Abstract
Objective:To explore the efficacy and feasibility of OECs graft in the treatment of old spinal cord injury. Methods:The wistar rats were prepared into the model of the T10 left side of the spinal cord hemisection injury. These rats were allowed for self-healing for 1 month without any treatment. When the recovery of the spinal function became slow and went into a plateau,CBS score was made,in which each rat was scoring 47. Then the rats were randomly divided into 3 groups(10 each group):vein graft group of olfactory ensheathing cells(Group A),D/ F12 cell culture medium graft group (Group B)and blank control group(Group C). The recovery of spinal function was evaluated by the CBS score and histo-logical examination. Results:According to the CBS scores,significant differences were shown between Group A and Group B/ C at each time point from the second week(P < 0. 05). No significant difference was shown between Group B and Group C(P > 0. 05). According to the microscopic observation of morphological changes in the spinal cord injury sites,signifi-cant differences were shown between Group A and Group B/ C. Group A had smaller scar tissues and more nerve fiber axon regeneration than Group B/ C,which showed better spinal cord recovery. Conclusion:The vein graft of olfactory ensheat-hing cells has a certain effect on the treatment of old spinal cord injury. This method is simple and easily-operated and will have good perspective for clinical practice and application.
NETL
NSTL
维普
万方数据