Protective effects of physcion on isoproterenol-induced myocardial injury
Objective:To explore the protective effects and underlying mechanism of physcion on isoproterenol(ISO)-induced myocardial injury.Methods:To establish the rat model of myocardial injury,rats were subcutaneously administered ISO and cardiac mass index was calculated.Enzyme-linked immunosorbent assay(ELISA)was used to detect the serum levels of creatine kinase-MB(CK-MB)and lactate dehydrogenase(LDH)in rats,and cardiac function was evaluated using echocardiography.H9c2 cells were incubated with ISO to induce a cell model of ISO-injured cardiomyocytes.Cell survival rate was measured using MTT assay,and protein expressions were detected using Western blotting.Results:In the ISO-induced rat model of myocardial injury,cardiac mass was increased,the serum levels of CK-MB and LDH were increased,and cardiac function indicators such as ejection fraction(EF)and fractional shortening(FS)were decreased.However,intervention with physcion or propranolol decreased the changes of above indicators,respectively.In the ISO-induced H9c2 cell injury model,physcion prevented ISO-induced decreases in cell viability,increases in LDH levels,and increases in the protein expression of TLR4,MyD88,NF-κB,and TNF-α.Conclusion:These results indicate that physcion is able to protect ISO-induced myocardial injury,which is related to the TLR4/MyD88/NF-κB signaling pathway.
万方数据
维普
