目的 研究信号转录和激活因子3(signal transduction and transcription activator3,STAT3)抑制剂WP1066对人气道上皮BEAS-2B细胞增殖的影响及分子机制,探讨WP1066治疗支气管哮喘疾病发病的潜在机制.方法 WP1066处理BEAS-2B细胞,应用MTT法测定细胞增殖,Western Blot 测定STAT3磷酸化水平及其下游靶点Cyclin D1表达水平.结果 WP1066可抑制BEAS-2B细胞的增殖,其抑制作用呈浓度依赖性,差异具有统计学意义(P<0.05);WP1066处理细胞并不会降低总的STAT3蛋白水平,但可抑制STAT3磷酸化水平,抑制下游Cyclin D1蛋白表达.结论 WP1066可通过阻断STAT3信号通路,从而抑制人气道上皮细胞增殖.
Effect and mechanism of WP1066 on human airway epithelial cell proliferation
Objective:To investigate the effect of signal transcription and activation factor 3(STAT3)inhibitor WP1066 on the proliferation of human airway BEAS-2B cells and explore the potential molecular mechanism of WP1066 in the treatment of bronchial asthma.Methods:After pretreatment with WP1066,the proliferation of BEAS-2B cells was determined by MTT assay.The level of STAT3 phosphorylation and its downstream target Cyclin D1 were measured by Western Blot.Results:WP1066 inhibited the proliferation of BEAS-2B cells in a concentration-dependent manner.WP1066 did not reduce the total STAT3 protein level,but inhibited the phosphorylation of STAT3 and down-regulated the expression of Cyclin D1 protein.Conclusion:WP1066 can inhibit the proliferation of human airway epithelial cells by blocking the STAT3 signaling pathway.
human airway epithelial cellproliferationWP1066STAT3Cyclin D1
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