Stress granules regulate cadmium-induced apoptosis of lung epithelial
Objective:This study aims to investigate the effect of stress granules(SGs)on cadmium-induced cell apoptosis in lung epithelial cells.Methods:Cadmium chloride-exposed lung epithelial cells were collected for PI/AnnexinV staining and then were detected by flow cytometry.Immunofluorescence assay was used to detect the formation of stress granules.Western blot assay was used to detect the expression level of Puromycin,eukaryotic translation initiation factor 2 subunit alpha(eIF2α)and phosphorylation of eIF2α(p-eIF2α).Results:Cadmium exposure can induce apoptosis of lung epithelial cells.Stress granules formation decreases cell apoptosis induced by cadmium exposure.The formation of stress granules is accompanied by phosphorylation of eIF2α.Inhibition of eIF2α phosphorylation inhibits the formation of stress granules.Conclusion:Phosphorylation of eIF2α-dependent stress granules could regulate cadmium-induced lung epithelial cell apoptosis.
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