Objective:To establish the quality standard of rape pollen granules.Methods:The flavonoids in rape pollen granules were qualitatively identified by TLC.Quercetin,nsringin,kaemoferol and isorhamnetin(Quercetin and other index components)were quantitatively analyzed by HPLC.Results:The spots of quercetin and kaempferol were clear and well separated by TLC.Quercetin and other index components showed a good linear relationship in the range of 20～320 mg/L(r=0.9999),4～64 mg/L(r=0.9993),60～960 mg/L(r=0.9999),0.5～8 mg/L(r=0.9993).The average recovery(RSD)were 96.7%(2.83%),102.7%(3.61%),98.2%(1.29%),and 98.9(4.43%).Conclusion:The method is simple,reliable and specific,which can be used for the quality control of rape pollen granules.
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