Bioinformatics analysis of differentially expressed genes in macrophage subsets of lung metastases
Objective:This article is to explore the role and mechanism of macrophage subsets in lung metastases,and to explore the differential genes and signaling pathways expressed by alveolar macrophages and interstitial macrophages in mouse lung metastases.Methods:Single-cell RNA sequencing(seRNAseq)data GSM5235892 were obtained from the gene expression database of mouse melanoma lung metastasis.Bioinformatic analysis techniques were used to screen potential differential genes of and to conduct gene ontology enrichment analysis,Kyoto Encyclopedia of Genes and Genes pathway analysis,protein interaction analysis and screening to identify the top 10 genes with high scores.Finally,the expression levels of core genes in mouse melanoma lung metastasis model alveolar macrophages and interstitial macrophages were detected by qPCR.Results:A total of 1 975 differentially expressed genes of alveolar macrophages and interstitial macrophages were identified,including 1 511 up-regulated genes and 464 down-regulated genes.Gene ontology enrichment analysis and kyoto encyclopedia of genes and genomes pathway analysis showed that the genes were mainly involved in immune response,tumor cell immune regulation,neuronal synaptic immune synapse,growth factor receptor activity,etc.The signaling pathways involved were NF-κB,TNF-α,MAPK,etc.The top 10 hub genes in PPI analysis were related to cellular oxidative phosphorylation,including Ndufa8,Cox6b1,Uqcr11,Cox5a,Ndufs6,Ndufs5,Uqcr10,Uqcrq,Ndufb6,Ndufab1;Through qPCR detection,we found that the expressions of hub gene Uqcr11,Ndufa5 were higher in alveolar macrophages and interstitial macrophages,which was consistent with the results of bioinformatics analysis.Survival analysis showed that the expression of the key gene Cox5a was associated with the survival of patients with skin melanoma.Conclusion:Through scRNAseq data bioinformatics analysis method,the key genes and signaling pathways expressed by alveolar macrophages and interstitial macrophages in mice with melanoma lung metastasis were screened so as to provide references for the study of the pathogenesis of melanoma lung metastasis.
万方数据
维普
