摘要
背景:黏蛋白6(MUC6)异常表达在多种炎症性疾病和癌症中具有重要意义,探讨其在反流性食管炎(RE)中的作用对疾病的早期诊治具有潜在价值.目的:探究MUC6与RE的相关性,为RE的早期诊治提供一定理论依据.方法:采用免疫组化技术检测MUC6在正常胃食管连接处(GEJ)组织、轻中度RE(洛杉矶分级A/B级)和重度RE(洛杉矶分级C/D级)以及无上皮内瘤变(GIN)、低级别上皮内瘤变(LGIN)和高级别上皮内瘤变(HGIN)GEJ组织中的表达情况,分析MUC6与RE临床特征的关系.结果:MUC6主要表达于细胞膜和细胞质,在正常GEJ组织中呈阴性或散在弱阳性表达,表达率为20.0％(2/10),在REGEJ组织中呈强阳性表达,表达率为100％(25/25).MUC6表达与性别、年龄、体质指数、Barrett食管之间无明显相关性(P均＞0.05);在无食管裂孔疝(HH)、HH直径＜3 cm、HH直径≥3 cm,正常、轻中度、重度RE,无GIN、LGIN、HGIN GEJ组织中的表达存在显著差异(P均＞0.05).结论:MUC6与RE严重程度呈正相关.
Abstract
Background:Aberrant expression of mucin 6(MUC6)is involved in multiple inflammatory diseases and cancers.Investigation on the role of MUC6 in reflux esophagitis(RE)is of great importance for early diagnosis and treatment of the disease.Aims:To investigate the relationship between MUC6 and RE,so as to provide theoretical basis for the early diagnosis and treatment of RE.Methods:Expression of MUC6 in normal gastroesophageal junction(GEJ)tissue,and GEJ tissues in mild-to-moderate RE(LA-A/B)and severe RE(LA-C/D),as well as in non-,low-,and high-grade intraepithelial neoplasia(non-GIN,LGIN and HGIN)was detected by immunohistochemistry.The relationship between MUC and the clinical futures of RE was analyzed.Results:MUC6 mainly expressed in the cell membrane and cytoplasm.Its expression was negative or weakly positive in normal GEJ tissue(20％,2/10)and strongly positive in GEJ tissues of RE patients(100％,25/25).MUC expression was not correlated with the gender,age,body mass index and Barrett esophagus(all P＞0.05).Significant correlations were observed between MUC6 expression and the hiatal hernia(HH;non-HH,HH＜3 cm and ≥3 cm in diameter),severity of RE(normal GEJ,mild-to-moderate RE,and severe RE),and intraepithelial neoplasia(non-GIN,LGIN,and HGIN)(all P＜0.05).Conclusions:MUC6 is positively correlated with the severity of RE.
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