银杏叶片对多巴胺神经元损伤的保护作用及机制
The neuroprotective effects and mechanism of Ginkgo biloba tablet on dopaminergic neuron damage
吴春艳 1刘国丹 1岳奎涛 2龚莉3
作者信息
- 1. 山东第二医科大学附属医院神经内科,山东潍坊 261031
- 2. 山东第二医科大学附属医院医学影像中心
- 3. 山东第二医科大学附属医院医务部
- 折叠
摘要
目的 探索银杏叶片对MPP+诱发的SH-SY5y细胞损伤的保护作用及对Nrf2/HO-1信号通路的影响.方法 采用MPP+孵育SH-SY5y细胞制备体外帕金森病细胞模型;予以银杏叶片含药血清干预,观察其对多巴胺能神经元损伤的改善作用及对Nrf2、HO-1、ROS水平的影响;采用Nrf2/HO-1信号通路特异性抑制剂干预,探索银杏叶片神经保护作用与Nrf2/HO-1信号通路的关系.结果 ①银杏叶片含药血清显著减轻了 MPP+诱发的SH-SY5y细胞损伤,升高了细胞Nrf2、HO-1的表达水平,抑制了 ROS生成(P＜0.05);②JNK、ERK抑制剂显著减弱了银杏叶片含药血清的细胞保护作用及抗氧化作用(P＜0.05);③P38抑制剂对银杏叶片含药血清的作用无显著影响(P＞0.05).结论 银杏叶片可有效改善MPP+诱发的SH-SY5y细胞损伤,机制与JNK、ERK途径介导的Nrf2/HO-1信号通路激活有关.
Abstract
Objective To investigate the protective effect of Ginkgo biloba tablet on MPP+-induced SH-SY5y cell damage and on the activity of Nrf2/HO-1 signaling pathway.Methods A cell model of Parkinson's disease(PD)was established by MPP+-stimulation fol-lowed by incubation with Ginkgo biloba tablet-containing serum in vitro.The effects of the drug-containing serum on the dopaminergic neuron damage as well as on the levels of Nrf2,HO-1 and ROS were examined.Additionally,specific inhibitors of the Nrf2/HO-1 signaling pathway were used to verify the correlation between the neuroprotective effect and the activity of Nrf2/HO-1 signaling pathway.Results Ginkgo biloba tablet-containing serum markedly attenuated the injury of the SH-SY5y cells induced by MPP+-stimulation,promoted the expression of Nrf2 and HO-1,and inhibited the ROS production from SH-SY5y cells.However,ERK inhibitor PD98059 and JNK inhibitor SP600125,but not P38 inhibitor SB203580,significantly abolished the neuroprotective effects and anti-oxidative actions of Ginkgo biloba tablet-containing serum in the SH-SY5y cells.Conclusion Ginkgo biloba tablet-containing serum can effectively ameliorate the SH-SY5y cell damage induced by MPP+-stimulation and the neuroprotective effects in the PD cell model might be associated with the activation of Nrf2/HO-1 signaling through JNK and ERK pathways.
关键词
银杏叶片/帕金森病/抗氧化作用/Nrf2/HO-1信号通路Key words
Ginkgo biloba tablet/Parkinson's disease/Antioxidant effect/Nrf2/HO-1 signaling pathway引用本文复制引用
基金项目
潍坊市卫生健康委第二类项目(2023)(WFZYY2023-1-003)
出版年
2024
NETL
NSTL
万方数据