Objective To investigate the difference and correlation between the expression of Ki-67 protein in metastatic lymph nodes and the primary breast tumor cells by immunocytochemistry reverse staining.Methods Sixty-one breast cancer patients with suspected axillary metastasis were collected from Weifang Hospital of Traditional Chinese Medicine.Ultrasound-guided fine-needle aspiration biopsy of the breast mass,coarse-needle aspiration biopsy of the pri-mary tumor and fine needle aspiration biopsy of suspected metastatic lymph nodes of imaging assessed were performed on these patients;then to evaluate the difference during these methods.The secondary analysis was performed in 45 patients with axillary lymph node metastasis confirmed via immunocytochemistry Ki-67 reverse staining.Results In 61 patients with breast cancer,the accuracy of fine-needle aspiration biopsy and coarse-needle aspiration biopsy was both 100％,but there was a slight difference in the classification of pathological types.In 45 patients with metastasis,the variation rate of Ki-67 in metastatic lymph nodes and primary lesions was 11.1％.When armpit/primary Ki-67 ratio was performed,there were statistical differences in the primary classification and pathological complete response(PCR)after neoadjuvant chemotherapy in breast cancer(P＜0.05),while there were no statistically significant differences in age,stage,estrogen receptor(ER),progestogen receptor(PR),and human epidermal growth factor receptor 2(HER-2)in primary breast cancer(P＞0.05).In the single-factor analysis of PCR after neoadjuvant chemotherapy,a significant correlation was wit-nessed between the Ki-67 of breast cancer[0.912 and 0.996(95％confidence interval)]and the Ki-67 ratio of axilla-ry/primary[7.651 and 677.567(95％confidence interval)](P＜0.05).In univariate analysis,variables with P＜0.1 were included in the multivariate logistic regression analysis.The results indicated that only the Ki-67 ratio[53.698(5.425-531.538)]of axillary lymph node/primary focus of breast cancer metastasis was related to the pCR after neoadju-vant chemotherapy(P＜0.05),while the Ki-67 ratio[0.976(0.920-1.035)]of primary focus of breast cancer was not significantly related to pCR after neoadjuvant chemotherapy(P＞0.05).Conclusion The expression of Ki-67 in breast cancer metastatic axillary lymph nodes/primary breast cancer may predict PCR after neoadjuvant chemotherapy.
Breast cancerAxilla metastatic lymph nodeFine-needle aspiration biopsyImmunocytochemis-tryKi-67
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