Aim at the indeterminate rationality of the evaluation criteria used in the comfort level evaluation of pedestri-an bridges,based on the simplified analysis model of MSD,the human-bridge coupling dynamic equation under the random action of many people was established.Through the probability statistical analysis of the results of many times numerical calculations,it was found that the root-mean-square acceleration as the evaluation criteria was more reasonable than the structural peak acceleration.Using the RMS(Root Mean Square)Acceleration as the evaluation criterion,the comfort level of a pedestrian bridge engineering case was evaluated by the improved annoyance rate model.The results show that the comfort level of a pedestrian bridge is mainly determined by the vibration threshold of the pedestrian,and is related to the pedestrian flow.The research results provide a reference for the comfort level design of pedestrian bridges.