Overexpression of miR-144-3p downregulates IL-6 mRNA in infected macrophages to inhibit BCG survival
To investigate the effect of miR-144-3p on macr-ophage IL-6 mRNA expression and macrophage immunocompetence,and to provide theore-tical reference for tuberculosis diagnosis and treatment.The method is establish a BCG-infected THP-1 macrophage model,qPCR to detect changes in miR-144-3p and IL-6 mRNA expression at different times of infection;transiently transfect miR-144-3p mimics/inhibitors to overpress/inhibiel miR-144-3p after the qPCR to detect miR-144-3p and IL-6 mRNA ex-pression changes at different times of infution;Pearson's correlation coefficient analysis the miR-144-3p and IL-6 mRNA corre-lation;CCK-8 to detect the proliferative capacity of BCG-infected macrophages after t-ransiently transfect-ing miR-144-3p was used mimics/inhibitors;and CFU was used to detect BCG survival stat-us.Both miR-144-3p and IL-6 mRNA expression increased significantly(P＜0.05)after ma-crophages were infected with BCG,with miR-144-3p expression peaking at 12 h,and IL-6 mRNA expression peaking at 24 h.Overexpres-sion of miR-144-3p after macrophages were infected with BCG resulted in a significant decrease(P＜0.01)in IL-6 mRNA expression;inhibition of miR-144-3p significantly increased IL-6 m-RNA expression(P＜0.01);there was a negative correlation between the two as analyzed by Pearson's correlation coefficient;overexpression/inhibition of miR-144-3p did not affect the proliferative capacity of macrophages,but over-expression of miR-144-3p inhibited the survival of BCG in macrophages.Both miR-144-3p and IL-6 mRNA expression were significantly increased in BCG-infected macrophages;overexpression/inhibition of miR-144-3p i-n macrophages and miR-144-3p may negatively regulate IL-6 mRNA expression;overexp-ression of miR-144-3p inhibits BCG survival and thus enhances the innate immunity of m-acrophages.
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