Mutagenic breeding and transcriptome analysis of Aurantiochytrium limacinum with high yield DHA production
Aurantiochytrium limacinum can accumulate large amounts of omega-3 polyunsaturated fatty acids,especially high levels of docosahexaenoic acid,which plays an important role in the food industry.In order to obtain a high DHA-producing strain,a mutant M2-11 with an 11.42％increase in DHA production was obtained in this study by electron-beam(EB)radiation mutagenesis combined with screening by mal-onate and nile red staining.The results of transcriptomics showed that the vast majority of genes related to lipid synthesis were up-regulated in M2-11 compared to the original strain,and several other functional genes with significant differences also existed.This study provides a reference for mutagenic selection and genetic modification of A.limacinum.
万方数据
维普
