Investigating the role of the zebrafish hypoxia-inducible factor-1 alpha(Hif-1α)gene in the devel-opment and migration of neutrophils and macrophages.Zebrafish were divided into wild-type control group and Hif-1α gene mutant group.CRISPR/Cas9 technology was utilized to mutate the Hif-1α gene in ze-brafish.Subsequently,Sudan Black B staining was performed to observe the staining of neutrophils in the wild-type control group and Hif-1α gene mutant group,and Neutral Red staining was employed to observe the staining of macrophages,followed by statistical analysis of the results.The results demonstrated suc-cessfully construction of zebrafish Hif-1α gene mutant with deletion of 8 bases.Furthermore,after 3 days of development in zebrafish larvae with mutated Hif-1α gene,there was a significant decrease in the number of neutrophils migrating to acute injury sites,while the number of microglial macrophages in the posterior ventricle of mutant zebrafish significantly increased compared to the wild-type.In summary,this study suc-cessfully constructed a zebrafish Hif-1α gene homozygous mutant.Additionally,it elucidated the role of Hif-1α gene in inhibiting neutrophil migration to inflammatory regions and promoting the growth and de-velopment of ventricular macrophages,laying a foundation for subsequent studies on acute injury models.
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