Construction and optimization of synthetic pathway of naringin from Saccharomyces cerevisiae
In this study,a naringenin cell synthesis plant of Saccharomyces cerevisiae was constructed to op-timize the key synthetic steps and increase naringenin production.A synthetic pathway from para-coumaric acid to naringin was constructed at the rDNA site of Saccharomyces cerevisiae using homologous recombi-nation technology,including a complete expression reading frame of 4-coumaric acid CoA ligase(4CL),Chalcone synthetase(CHS)and Chalcone isomerase(CHI).At the same time,the single factor method was used to optimize the yeast extract in YPD fermentation medium.Glucose and tryptone concentrations.The results showed that 5 strains S1,S2,S3,S4 and S5 of S.cerevisiae producing naringin were obtained.The maximum naringin yield was 438mg/L through batch feeding fermentation under the optimal ratio of 4％yeast extract,10％tryptonate and 2％glucose.The construction and optimization of heterologous syn-thesis pathway of naringenin in Saccharomyces cerevisiae were successfully realized,which provided a new way and reference value for industrial microbial synthesis of naringenin.
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