中西药联用对黑斑蛙源"歪头病"病原菌的体外抑菌效果研究

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中文摘要：为研究常用抗生素和中草药对黑斑蛙源"歪头病"病原菌米尔伊丽莎白菌和肺炎克雷伯菌的体外抑菌效果,采用纸片扩散法和二倍稀释法测定了米尔伊丽莎白菌和肺炎克雷伯菌对9种常用抗生素和6种中草药的敏感性,棋盘法测定两联药方的部分抑菌浓度(FIC).结果显示,阿米卡星、多粘菌素B和环丙沙星对米尔伊丽莎白菌和肺炎克雷伯菌的抑菌效果较好,最小抑菌浓度(MIC)分别为2.5 µg/mL、5-10 μg/mL和10 µg/mL;五倍子、金诃子和金银花抑菌效果较好,MIC分别为31.25 mg/mL、125 mg/mL和500 mg/mL;这三种抑菌效果较好的中草药之间两两联用未发生拮抗作用.中西两联药方中,五倍子和多粘菌素B对米尔伊丽莎白菌的抑菌效果较好,FIC为1,表现为相加作用,可作为黑斑蛙"歪头病"抑菌药物的参考配伍组合.

外文标题：The in vitro anti-bacterial effect of Chinese herbal medicines and antibiotics and their compounds on pathogens caused torticollis in black-spotted frogs

外文摘要：To investigate the in vitro antibacterial effects of commonly used antibiotics and traditional Chinese medi-cines on the pathogenic bacteria caused torticollis in black-spotted frogs,the sensitivity of Elizabethkingia miricola and Klebsiella pneumoniae to 9 commonly used antibiotics and 6 traditional Chinese medicines was determined using the disk diffusion method and the double dilution method.The fractional inhibitory concentration(FIC)of two-drug combinations was further measured using the checkerboard method.The results showed that Amikacin,Polymyxin B,and Ciprofloxacin had better antibacterial effects against both pathogens,with minimum inhibitory concentrations(MIC)of 2.5 μg/mL,5-10 μg/mL,and 10 μg/mL,respectively;Rhus chinensis Mill.,Terminalia chebula Retz.,and Lonicerae japonicae had good antibacterial effects,with MIC values of 31.25 mg/mL,125 mg/mL,and 500 mg/mL,respectively.These three traditional Chinese medicines did not show antagonism when used in combination.A-mong the combinations between Chinese medicines and antibiotics,R.chinensis Mill.and Polymyxin B had a good antibacterial effect on E.miricola,with an FIC of 1,indicating an additive effect.This combination could be served as a reference for treatment of torticollis in black-spotted frogs.

外文关键词：

Rana nigromaculataTorticollisElizabethkingia miricolaKlebsiella pneumoniaeCompoundAntibacterial

作者：

黄美平、汪鑫、梅凯华、袁庆云、陈展鹏、熊勇、曹叙洲、万晓玲、陈楠

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作者单位：

武汉轻工大学动物科学与营养工程学院,武汉 430023

黄冈市农业科学院,黄冈 438000

湖北隆润农业科技发展有限公司,黄冈 438000

关键词：

黑斑蛙歪头病米尔伊丽莎白菌肺炎克雷伯菌复方抑菌

基金：

湖北省自然科学基金青年项目武汉市农科院创新体系科技强链项目黄冈市2023年度青年拔尖人才培养计划武汉轻工大学博士启动项目武汉轻工大学校级"大学生创新创业训练计划项目"

项目编号：

2023AFB031DSQ2023042023HQB062024RZ047202310496092

出版年：

2024

DOI：

10.3969/j.issn.2095-7386.2024.05.001

武汉轻工大学学报

武汉工业学院

武汉轻工大学学报

影响因子：0.356

ISSN：1009-4881

年,卷(期)：2024.43(5)