The in vitro anti-bacterial effect of Chinese herbal medicines and antibiotics and their compounds on pathogens caused torticollis in black-spotted frogs
The in vitro anti-bacterial effect of Chinese herbal medicines and antibiotics and their compounds on pathogens caused torticollis in black-spotted frogs
To investigate the in vitro antibacterial effects of commonly used antibiotics and traditional Chinese medi-cines on the pathogenic bacteria caused torticollis in black-spotted frogs,the sensitivity of Elizabethkingia miricola and Klebsiella pneumoniae to 9 commonly used antibiotics and 6 traditional Chinese medicines was determined using the disk diffusion method and the double dilution method.The fractional inhibitory concentration(FIC)of two-drug combinations was further measured using the checkerboard method.The results showed that Amikacin,Polymyxin B,and Ciprofloxacin had better antibacterial effects against both pathogens,with minimum inhibitory concentrations(MIC)of 2.5 μg/mL,5-10 μg/mL,and 10 μg/mL,respectively;Rhus chinensis Mill.,Terminalia chebula Retz.,and Lonicerae japonicae had good antibacterial effects,with MIC values of 31.25 mg/mL,125 mg/mL,and 500 mg/mL,respectively.These three traditional Chinese medicines did not show antagonism when used in combination.A-mong the combinations between Chinese medicines and antibiotics,R.chinensis Mill.and Polymyxin B had a good antibacterial effect on E.miricola,with an FIC of 1,indicating an additive effect.This combination could be served as a reference for treatment of torticollis in black-spotted frogs.
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