Induction of callus and suspension cell culture in Welwitschia mirabilis Hook.f.
To explore a stable and efficient system for inducing callus tissue from leaves of Welwitschia mirabilis and suspension cell culture method,using leaves of Welwitschia mirabilis as materials,plant culture medium was pre-pared by orthogonal method with two hormones(NAA and 6-BA).Two treatments,light avoidance and circadian rhythm,were used to induce callus formation,and suspension cells were cultured using the corresponding ratio of callus tissue and liquid culture medium.The results indicate that induction of callus from leaves of Welwitschia mir-abilis at the concentration of 0.5 mg/L 6-BA+1.0 mg/L NAA showed the best stability and success rate,and the efficiency of induction and growth under circadian rhythm conditions was very high.Suspension cells cultured on this basis have made certain progress.The research can provide technical reference for the protection and stable in-heritance of Welwitschia mirabilis.
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