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苹果酸协同茶多酚对面包抗老化和消化性质的影响

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面包在贮藏过程中,茶多酚能缓解淀粉回生老化导致的水分降低、硬化度增加等品质劣变现象,但是蛋白质-茶多酚相互作用存在不稳定性、阻碍淀粉消化的问题.本文旨在研究苹果酸(malic acid,MA)对面筋蛋白(gluten proteins,GP)与茶多酚(tea polyphenol,TP)之间相互作用的改善,并对面包老化和消化性质进行研究.采用快速黏度仪研究面粉糊化性质;通过低场核磁共振成像仪探究贮藏期间面包水分含量和水分分布的变化规律;利用差示扫描量热仪研究淀粉在贮藏过程中的回生行为;通过荧光光谱分析研究苹果酸对茶多酚分别与面筋蛋白和酶蛋白之间相互作用的荧光猝灭作用以及对淀粉消化的影响.研究结果表明,苹果酸的添加促进了复合物的稳定性,使复合物显著降低了淀粉的回生焓值、减缓了面包水分的减少速率和延缓了面包硬化速度.同时,苹果酸也改善了茶多酚对α-淀粉酶活性抑制作用.为苹果酸协同茶多酚-蛋白复合物作用于淀粉类谷物食品提供了一种新思路.
Effects of malic acid and tea polyphenols on anti-aging and digestive properties of bread
During the storage of bread,tea polyphenols can alleviate the deterioration of quality caused by starch ret-rogradation,such as the decrease of moisture and the increase of hardening degree,but the interaction between pro-tein and tea polyphenols is unstable and hinders starch digestion.The purpose of this study was to study the effects of malic acid(MA)on the interaction between gluten proteins(GP)and tea polyphenols(TP),and to study the ag-ing and digestive quality of bread.The gelatinization properties of flour were studied by rapid viscometer,the chan-ges of moisture content and water distribution of bread during storage were studied by low-field nuclear magnetic resonance imaging,and the retrogradation behavior of starch during storage was studied by differential scanning calo-rimetry.In addition,Fluorescence quenching of the interaction between tea polyphenols and gluten protein and en-zyme protein and starch digestion were studied by fluorescence spectrum analysis.The results showed that the addi-tion of malic acid promoted the stability of the complex,significantly reduced the retrogradation enthalpy of starch,slowed down the decreasing rate of bread moisture and delayed the bread hardening rate.At the same time,malic acid also improved the inhibition of tea polyphenols on α-amylase activity.It can provide a new idea for malic acid combined with tea polyphenol protein complex to act on starch cereals.

malic acidgluten proteinstea polyphenolsinteraction mechanismbreadagingdigestion

冯如意、龚欣怡、周连、张海铭、张海枝、刘刚、秦新光

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武汉轻工大学食品科学与工程学院,武汉 430023

武汉轻工大学农产品加工与转化湖北省重点实验室,武汉 430023

苹果酸 面筋蛋白 茶多酚 相作机制 面包 老化 消化

2024

武汉轻工大学学报
武汉工业学院

武汉轻工大学学报

影响因子:0.356
ISSN:1009-4881
年,卷(期):2024.43(6)