Screening of a 2,6-dimethylphenol degrading bacterium and study on its degradation pathway
The compound 2,6-xylenol is a highly toxic and potential carcinogenic alkylphenol pollutant.However,the mechanisms of its degradation remain unknown.Here,we report the isolation of Pseudomonas sp.26421 from a wastewater treatment factory.It could utilize 2,6-xylenol,gentisate,protocatechuate,but not catechol or other xy-lenol isomers,as sources of carbon and energy.Pseudomonas sp.26421 completely degraded 2.5 mmol/L 2,6-xy-lenol within 108 hours.Intact cells of Pseudomonas sp.26421 grew with 2,6-xylenol co-oxidized 2,6-xylenol to 2,6-dimethyl hydroquinone,which was quantitatively converted to 2,6-dimethyl-p-benzoquinone that could be accu-rately identified by HPLC and GC-MS.These results indicate that the degradation of 2,6-xylenol in strain 26421 begins not with the oxidation of methyl group,but from hydroxyl oxidation of the benzene ring.
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