原位制备ZIF-8/纤维素微球及其控制释放5-氟尿嘧啶的研究
ZIF-8/cellulose microspheres prepared in situ to control release of 5-fluorouracil
郭远哲 1毛思颖 1罗晓刚2
作者信息
- 1. 武汉工程大学化工与制药学院,绿色化工过程教育部重点实验室(武汉工程大学),湖北 武汉 430205
- 2. 武汉工程大学化工与制药学院,绿色化工过程教育部重点实验室(武汉工程大学),湖北 武汉 430205;湖北三峡实验室,湖北 宜昌 443007
- 折叠
摘要
在纤维素微球(CM)上设计了沸石型咪唑骨架材料 8/纤维素复合微球(ZIF-8@CM)作为 5-氟尿嘧啶(5-FU)的载体并对 5-FU进行控制释放.以CM、硝酸锌和 2-甲基咪唑为原料,采用原位法制备了ZIF-8@CM.通过氢键、静电相互作用和π-π堆积作用将 5-FU加载到ZIF-8@CM上.利用扫描电子显微术、能量色散X射线分析法、傅里叶变换红外光谱法、X射线衍射、X射线光电子能谱法和热重分析对样品进行表征,结果表明成功制备了ZIF-8@CM,并在ZIF-8@CM上成功加载了5-FU.ZIF-8@CM对5-FU的负载量可达 74.10 mg·g-1,远高于CM(2.84 mg·g-1).此外,ZIF-8@CM-5-FU可在pH=5.0 的磷酸缓冲溶液(PBS)环境下持续释放 5-FU 600 min,释放量达到 63.4%,但在pH=7.4的PBS环境下仅释放 37.6%.5-FU的释放遵循一级动力学和Fickian扩散机制.在细胞实验中已证实ZIF-8@CM具有良好的生物相容性和极低的细胞毒性,是5-FU安全释放的合适载体.本研究为设计一种5-FU的新型载体提供了思路.
Abstract
Zeolitic imidazole framework 8/cellulose composite microsphere(ZIF-8@CM)was designed on the cellulose microsphere(CM)as the carrier of 5-fluorouracil(5-FU)to control the release of 5-FU.ZIF-8@CM was prepared by an in-situ method from CM,zinc nitrate and 2-methylimidazole.5-FU was loaded on ZIF-8@CM by hydrogen bonding,electrostatic interaction and π-π stacking.The samples were characterized by scanning electron microscopy,energy-dispersion X-ray analysis,Fourier transform infrared spectroscopy,X-ray diffraction,X-ray photoelectron spectroscopy and thermogravimetric analysis.The results show that ZIF-8@CM is successfully prepared and 5-FU is loaded on ZIF-8@CM.The loading capacity of 5-FU can reach 74.10 mg·g-1,much higher than that of CM(2.84 mg·g-1).In addition,ZIF-8@CM-5-FU can release 63.4%of 5-FU over 600 min in phosphoric acid buffer solution(PBS)at pH=5.0,but only 37.6%in PBS at pH=7.4.The release of 5-FU follows first-order kinetics and Fickian diffusion mechanism.Cell experiments prove that ZIF-8@CM have good biocompatibility and very low cytotoxicity,and ZIF-8@CM-5-FU is a suitable vector for safe release of 5-FU.This study provides an idea for designing a new type of 5-FU carrier.
关键词
纤维素微球/ZIF-8/5-氟尿嘧啶/控制释放Key words
cellulose microspheres/ZIF-8/5-fluorouracil/controlled release引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据