Corruption Shewanella putrefaciens 4H Chromium Reduction Mechanism of Exploration
In order to investigate the mechanism of Cr(Ⅵ)reduction by S.putrefaciens 4H,scanning electron microscopy(SEM),Fourier-transform infrared spectroscopy(FTIR),X-ray photoelectron spectroscopy(XPS)and electrochemical testing were used to characterize it.The results indicate that the mechanism of Cr(Ⅵ)reduction by S.putrefaciens 4H was mainly related to the metabolism and electron trans-port capacity of microorganisms.XPS analysis revealed that there were two forms of Cr(Ⅵ)and Cr(Ⅲ)on the surface of bacteria during the reduction of Cr(Ⅵ),which further proved that the bacteria removed hexavalent chromium by reduction method.SEM results showed that there was no obvious damage on the surface of the bacteria under chromium hexavalent stress,and the morphology of the bacteria changed from short rod type to long rod type,indicating that the bacteria had strong tolerance to Cr(Ⅵ).FTIR results show that with the increase of initial Cr(Ⅵ)concentration,the characteristic peaks represented by hydroxyl,carboxyl,amide and phosphate groups also increase signifi-cantly.The results of electron transfer performance test and electrochemical test show that the bacterium has strong electron transfer ability.This study provides a theoretical basis for the microbial treatment of Cr(Ⅵ)in reduction environments by investigating the mechanism of Cr(Ⅵ)reduction by S.putrefaciens4H.
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