首页|Allelotyping for loss of heterozygosity on chromosome 18 in gastric cancer

Allelotyping for loss of heterozygosity on chromosome 18 in gastric cancer

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AIM: To investigate the association between loss of heterozygosity (LOH) on chromosome 18 and sporadic gastric cancer.METHODS: Multiplex PCR was used to screen 14 highly polymorphic microsatellite markers on chromosome 18 in45 cases of primary gastric cancer. PCR products were separated on polyacrylamide gels and the electrophoresis maps were analyzed with Genescan and Genotyper.RESULTS: The LOH frequencies in gastric cancer at all 14markers ranged from 10% to 58%. Eleven markers were found with over 20% LOH frequencies, in which 9 markers located in 18q, and 2 markers in 18p. Two overlapping deleted regions were identified: R1 between D18S61-D18S1161 at 18q22 (9cM) with 24% LOH frequency; R2 between D18S462-D18S70 at 18q22-23(6cM) with 32% LOH frequency.CONCLUSION: LOH of chromosome 18 (18q and 18p) may be involved in gastric tumorigenesis. Two overlapping deleted fragments suggested that there might be unidentified tumor suppressor genes in those two regions.

Jing-Cui Yu、Kai-Lai Sun、Buo Liu、Song-Bin Fu

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Department of Clinical Pharmacology,the Second Affiliated Hosptial,Harbin Medical University,Harbin 150086,Heilongjiang Province,China

Department of Medical Genetics,China Medical University,Shenyang 110001,Liaoning Province,China

Chinese National Human Genome Center,Beijing 100176,China

Laboratory of Medical Genetics,Harbin Medical University

Bio-pharmaceutical Key Laboratory of Heilongjiang Province,Harbin 150086,Heilongjiang Province,China

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国家自然科学基金黑龙江省科研项目

30370783GB03C601-1

2004

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

CSTPCDSCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2004.10(13)
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