首页期刊导航|世界胃肠病学杂志（英文版）
期刊信息/Journal information
世界胃肠病学杂志（英文版）
世界胃肠病学杂志（英文版）

潘伯荣

周刊

1007-9327

wjg@wjgnet.com

010-85381901-628

100025

北京市朝阳区东四环中路62号楼远洋国际中心D座903室

世界胃肠病学杂志（英文版）/Journal World Journal of GastroenterologyCSCDCSTPCDSCI
查看更多>>主要报道和刊登国内外、特别是我国消化病学者具有创造性的、有较高学术水平的基础和临床研究论文、研究快报等. 对具有中国特色的研究论文, 如食管癌、胃癌、肝癌、大肠癌、病毒性肝炎、幽门螺杆菌、中医中药、中西医结合和基于作者自己研究工作为主的综述性论文, 将优先发表. 读者对象为基础研究或临床研究的消化专业工作者。
正式出版
收录年代

    Matrix metalloproteinase-9: A deleterious link between hepatic ischemia-reperfusion and colorectal cancer

    Sébastien LengletFran(c)ois MachFabrizio Montecucco
    7131-7133页
    查看更多>>摘要：Despite the advent of improved surgical techniques and the development of cytotoxic chemotherapeutic agents useful for the treatment of colorectal cancer,the primary clinical challenge remains that of preventing and combating metastatic spread.Surgical resection is the best treatment for colorectal metastases isolated to the liver.However,in rodent models,the hepatic ischemia-reperfusion (I/R) applied during the surgery accelerates the outgrowth of implanted tumors.Among the adverse effects of I/R on cellular function,several studies have demonstrated an over expression of the matrix metalloproteinase-9 (MMP-9) in the ischemic liver.Since several studies showed high local levels of expression and activity of this proteolytic enzyme in the primary colorectal adenocarcinoma,the role of MMP-9 might be considered as a potential common mediator,favoring both growth of local tumor and the dissemination of colorectal carcinoma metastases.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Carcinoma of the gastroesophageal junction in Chinese patients

    Qin Huang
    7134-7140页
    查看更多>>摘要：Carcinoma of the gastroesophageal junction (GEJ) is defined as carcinoma that crosses the GEJ line,irrespective of where the tumor epicenter is located.This group of cancer is rare but controversial.Based on study results from the majority of epidemiologic and clinicopathologic investigations carried out in Western countries,this cancer is believed to arise from Barrett's esophagus (BE) and includes both distal esophageal and proximal gastric carcinomas because of similar characteristics in epidemiology,clinicopathology,and molecular pathobiology in relation to BE.As such,the most recent American Joint Committee on Cancer staging manual requires staging all GEJ carcinomas with the rule for esophageal adenocarcinoma (EA).This mandate has been challenged recently by the data from several studies carried out mainly in Chinese patients.The emerging evidence derived from those studies suggests:(1) both BE and EA are uncommon in the Chinese population; (2) almost all GEJ cancers in Chinese arise in the proximal stomach and show the features of proximal gastric cancer,not those of EA; (3) application of the new cancer staging rule to GFJ cancer of Chinese patients cannot stratify patients' prognosis effectively; and (4) prognostic factors of GEJ cancer in Chinese are similar,but not identical,to those of EA.In conclusion,the recent evidence suggests that GFJ cancer in Chinese shows distinct clinicopathologic characteristics that are different from EA.Further investigations in molecular pathology may help illustrate the underlying pathogenesis mechanisms of this cancer in Chinese patients and better manage patients with this fatal disease.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Role of autoimmunity in primary biliary cirrhosis

    Tian-Yan ShiFeng-Chun Zhang
    7141-7148页
    查看更多>>摘要：Primary biliary cirrhosis (PBC) is an autoimmune liver disease characterized by the presence of serum autoantibodies and chronic nonsuppurative destructive cholangitis.The pathogenesis of PBC involves environmental factors,genetic predisposition and loss of immune tolerance.In recent years,it has become univocally accepted that an inappropriately activated immune response is one of the most important factors in PBC.In this study,the role of autoimmunity in PBC is summarized and a feasible research orientation is recommended.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Diagnostic and therapeutic progress of multi-drug resistance with anti-HBV nucleos(t)ide analogues

    Zhuo-Lun SongYu-Jun CuiWei-Ping ZhengDa-Hong Teng...
    7149-7157页
    查看更多>>摘要：Nucleos(t)ide analogues (NA) are a breakthrough in the treatment and management of chronic hepatitis B.NA could suppress the replication of hepatitis B virus (HBV) and control the progression of the disease.However,drug resistance caused by their long-term use becomes a practical problem,which influences the long-term outcomes in patients.Liver transplantation is the only choice for patients with HBV-related end-stage liver disease.But,the recurrence of HBV after transplantation often caused by the development of drug resistance leads to unfavorable outcomes for the recipients.Recentiy,the multi-drug resistance (MDR) has become a common issue raised due to the development and clinical application of a variety of NA.This may complicate the antiviral therapy and bring poorly prognostic outcomes.Although clinical evidence has suggested that combination therapy with different NA could effectively reduce the viral load in patients with MDR,the advent of new antiviral agents with high potency and high genetic barrier to resistance brings hope to antiviral therapy.The future of HBV researches relies on how to prevent the MDR occurrence and develop reasonable and effective treatment strategies.This review focuses on the diagnostic and therapeutic progress in MDR caused by the anti-HBV NA and describes some new research progress in this field.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Gardenia jasminoides attenuates hepatocellular injury and fibrosis in bile duct-ligated rats and human hepatic stellate cells

    Ying-Hua ChenTian LanJing LiChun-Hui Qiu...
    7158-7165页
    查看更多>>摘要：AIM:To investigate the anti-hepatofibrotic effects of Gardenia jasminoides in liver fibrosis.METHODS:Male Sprague-Dawley rats underwent common bile duct ligation (BDL) for 14 d and were treated with Gardenia jasminoides by gavage.The effects of Gardenia jasminoides on liver fibrosis and the detailed molecular mechanisms were also assessed in human hepatic stellate cells (LX-2) in vitro.RESULTS:Treatment with Gardenia jasminoides decreased serum alanine aminotransferase (BDL vs BDL +100 mg/kg Gardenia jasminoides,146.6 ± 15 U/L vs 77± 6.5 U/L,P =0.0007) and aspartate aminotransferase (BDL vs BDL + 100 mg/kg Gardenia jasminoides,188 ± 35.2 U/L vs 128 ± 19 U/L,P =0.005) as well as hydroxyproline (BDL vs BDL + 100 mg/kg Gardenia jasminoides,438 ± 40.2 μg/g vs 228 ± 10.3 μg/g liver tissue,P =0.004) after BDL.Furthermore,Gardenia jasminoides significantly reduced liver mRNA and/or protein expression of transforming growth factor β1(TGF-β1),collagen type Ⅰ (Col Ⅰ) and α-smooth muscle actin (α-SMA).Gardenia jasminoides significantly suppressed the upregulation of TGF-β1,Col Ⅰ and α-SMA in LX-2 exposed to recombinant TGF-β1.Moreover,Gardenia jasminoides inhibited TGF-β1-induced Smad2 phosphorylation in LX-2 cells.CONCLUSION:Gardeniajasminoides exerts antifibrotic effects in the liver fibrosis and may represent a novel antifibrotic agent.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Oridonin induces apoptosis in gastric cancer through Apaf-1, cytochrome c and caspase-3 signaling pathway

    Ke-Wang SunYing-Yu MaTian-Pei GuanYing-Jie Xia...
    7166-7174页
    查看更多>>摘要：AIM:To investigate the effect and mechanism of oridonin on the gastric cancer cell line HGC-27 in vitro.METHODS:The inhibitory effect of oridonin on HGC-27 cells was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay.After treatment with 10 μg/mL oridonin for 24 h and 48 h,the cells were stained with acridine orange/ethidium bromide.The morphologic changes were observed under an inverted fluorescence microscope.DNA fragmentation (a hallmark of apoptosis) and lactate dehydrogenase activity were examined using DNA ladder assay and lactate dehydrogenase-release assay.After treated with oridonin (0,1.25,2.5,5 and 10 μg/mL),HGC-27cells were collected for anexin V-phycoerythrin and 7-amino-actinomycin D double staining and tested by flow cytometric analysis,and oridonin-induced apoptosis in HGC-27 cells was detected.After treatment with oridonin for 24 h,the effects of oridonin on expression of Apaf-1,Bcl-2,Bax,caspase-3 and cytochrome c were also analyzed using reverse-transcript polymerase chain reaction (RT-PCR) and Western blotting.RESULTS:Oridonin significantly inhibited the proliferation of HGC-27 cells in a dose-and time-dependent manner.The inhibition rates of HGC-27 treated with four different concentrations of oridonin for 24 h (1.25,2.5,5 and 10 μg/mL) were 1.78％ ± 0.36％,4.96％ ±1.59％,10.35％ ± 2.76％ and 41.6％ ± 4.29％,respectively,which showed a significant difference (P ＜ 0.05).The inhibition rates of HGC-27 treated with oridonin at the four concentrations for 48 h were 14.77％ ± 4.21％,21.57％ ± 3.75％,30.31％ ± 4.91％ and 61.19％ ±5.81％,with a significant difference (P ＜ 0.05).The inhibition rates of HGC-27 treated with oridonin for 72 h at the four concentrations were 25.77％ ± 4.85％,31.86％ ± 3.86％,48.30％ ± 4.16％ and 81.80％ ± 6.72％,with a significant difference (P ＜ 0.05).Cells treated with oridonin showed typical apoptotic features with acridine orange/ethidium bromide staining.After treatment with oridonin,the cells became round,shrank,and developed small buds around the nuclear membrane while forming apoptotic bodies.Lactate dehydrogenase (LDH) release assay showed that after treated with 1.25 μg/mL and 20 μg/mL oridonin for 24 h,LDH release of HGC-27 caused by apoptosis increased from 22.94％ ± 3.8％ to 52.68％ ± 2.4％ (P ＜ 0.001).However,the change in the release of LDH caused by necrosis was insignificant,suggesting that the major cause of oridonin-induced HGC-27 cell death was apoptosis.Flow cytometric analysis also revealed that oridonin induced significant apoptosis compared with the controls (P ＜ 0.05).And the apoptosis rates of HGC-27 induced by the four different concentrations of oridonin were 5.3％ ± 1.02％,12.8％ ± 2.53％,28.5％ ± 4.23％ and 49.6％ ± 3.76％,which were in a dose-dependent manner (P ＜ 0.05).After treatment for 24 h,DNA ladder showed that oridonin induced a significant increase in DNA fragmentation in a dosedependent manner.RT-PCR revealed that mRNA expression levels were up-regulated compared with the controls in caspase-3 (0.917 ± 0.103 vs 0.357 ± 0.019,P ＜ 0.05),cytochrome c (1.429 ± 0.111 vs 1.002 ±0.014,P ＜ 0.05),Apaf-1 (0.688 ± 0.101 vs 0.242 ±0.037,P ＜ 0.05) and Bax (0.856 ± 0.101 vs 0.278 ±0.027,P ＜ 0.05) (P ＜ 0.05),whereas down-regulated in Bd-2 (0.085 ± 0.012 vs 0.175 ± 0.030,P ＜ 0.05).Western blotting analysis also confirmed this result.CONCLUSION:Apoptosis of HGC-27 induced by oridonin may be associated with differential expression of Apaf-1,caspase-3 and cytochrome c,which are highly dependent upon the mitochondrial pathway.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Age-related symptom and life quality changes in women with irritable bowel syndrome

    Yu-Rong TangWei-Wei YangMei-Lan LiangXin-Yu Xu...
    7175-7183页
    查看更多>>摘要：AIM:To explore age-related changes in symptoms and quality of life (QoL) of women with irritable bowel syndrome (IBS).METHODS:Two-hundred and fifty-four female adult outpatients with IBS attending the Department of Gastroenterology at the First Affiliated Hospital of Nanjing Medical University between January,2008 and October,2008 were approached.Patients with a history of abdominal surgery,mental illness or those who had recently taken psychotropic drugs were excluded.A physician obtained demographic and abdominal symptom data.All patients were asked to complete the Zung Self-Rated Anxiety and Depression Scale (SDS/SAS) and the IBS-specific QoL questionnaire.The patients were divided into six groups according to age,in 10-year increments:18-27 years,28-37 years,38-47 years,48-57 years,58-67 years and 68-75 years (maximum 75 years).Age-related differences of abdominal pain or discomfort were analyzed using ranksum tests.Differences in SDS/SAS and IBS-QoL scores between age groups were analyzed using one-way analysis of variance.Pearson's correlations evaluated potential associations between IBS symptoms,psychological factors and QoL in each age group.RESULTS:There were no differences in the distribution of IBS subtypes between age groups (x2 =20.516,P =0.153).Differences in the severity of abdominal pain/discomfort with age were statistically significant (x2 =25.638,P ＜ 0.001); patients aged 48-57 years,58-67 years or 68-75 years had milder abdominal pain/discomfort than those in the younger age groups.The severity of anxiety or depressive symptoms did not differ between age groups (SDS,x2 =390.845,P =0.110;SAS,x2 =360.071,P =0.220).Differences of IBSQoL scores were statistically significant between age groups (x2 =1098.458,P =0.011).The scores of patients in the 48-57-year group were lower than those in the 18-27-year and 28-37-year groups (48-57-year group vs 18-27-year group,74.88 ± 8.76 vs 79.76± 8.63,P =0.021; 48-57-year group vs 28-37-year group,74.88 ± 8.76 vs 79.04 ± 8.32,P =0.014).The scores in the 68-75-year group were lower than those in the 18-27-year,28-37-year and 38-47-year groups (68-75-year group w 18-27-year group,71.98± 9.83 w 79.76 ± 8.63,P =0.003; 68-75-year group vs 28-37-year group,71.98 ± 9.83 w 79.04 ± 8.32,P =0.002; 68-75-year group vs 38-47-year group,71.98 ± 9.83 vs 76.44 ± 8.15,P =0.039).Anxiety and depression were negatively correlated with QoL in all age groups (SDS and QoL:18-27-year group,r =-0.562,P =0.005; 28-37-year group,r =-0.540,P ＜ 0.001;38-47-year group,r =-0.775,P ＜ 0.001; 48-57-year group,r =-0.445,P =0.001; 58-67-year group,r =-0.692,P ＜ 0.001; 68-75-year group,r =-0.732,P ＜0.001.SAS and QoL:18-27-year group,r =-0.600,P =0.002; 28-37-year group,r =-0.511,P ＜ 0.001;38-47-year group,r =-0.675,P ＜ 0.001; 48-57-year group,r =-0.558,58-67-year group,P =0.001; r =-0.588,P ＜ 0.001; 68-75-year group,r =-0.811,P＜ 0.001).A negative correlation between abdominal pain severity and QoL was found in patients aged more than 58 years (58-67-year group,r =-0.366,P =0.017; 68-75-year group,r =-0.448,P =0.048),but not in younger patients (18-27-year group,r =0.080,P =0.716; 28-37-year group,r =-0.063,P =0.679;38-47-year group,r =-0.029,P =0.812; 48-57-year group,r =-0.022,P =0.876).CONCLUSION:Factors affecting QoL should always be treated in IBS,especially emotional problems in young adults.Even mild abdominal pain should be controlled in elderly patients.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Mechanisms of cholecystokinin-induced calcium mobilization in gastric antral interstitial cells of Cajal

    Yao-Yao GongXin-Min SiLin LinJia Lu...
    7184-7193页
    查看更多>>摘要：AIM:To investigate the effect of sulfated cholecystokinin-8 (CCK-8S) on calcium mobilization in cultured murine gastric antral interstitial cells of Cajal (ICC) and its possible mechanisms.METHODS:ICC were isolated from the gastric antrum of mice and cultured.Immunofluorescence staining with a monoclonal antibody for c-Kit was used to identify ICC.The responsiveness of ICC to CCK-8S was measured using Fluo-3/AM based digital microfluorimetric measurement of intracellular Ca2+ concentration ([Ca2+]i).A confocal laser scanning microscope was used to monitor [Ca2+]i changes.The selective CCK1 receptor antagonist lorglumide,the intracellular Ca2+-ATPase inhibitor thapsigargin,the type Ⅲ inositol 1,4,5-triphosphate (InsP3) receptor blocker xestospongin C and the L-type voltage-operated Ca2+ channel inhibitor nifedipine were used to examine the mechanisms of [Ca2+]i elevation caused by CCK-8S.Immunoprecipitation and Western blotting were used to determine the regulatory effect of PKC on phosphorylation of type Ⅲ InsP3 receptor (InsP3R3) in ICC.Protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) and inhibitor chelerythrine were used to assess the role of PKC in the CCK-8S-evoked [Ca2+]i increment of ICC.RESULTS:ICC were successfully isolated from the gastric antrum of mice and cultured.Cultured ICC were identified by immunofluorescence staining.When given 80 nmol/L or more than 80 nmol/L CCK-8S,the [Ca2+]i in ICC increased and 100 nmol/L CCK-8S significantly increased the mean [Ca2+]i by 59.30％ ± 4.85％ (P ＜0.01).Pretreatment of ICC with 5 μmol/L lorglumide inhibited 100 nmol/L CCK-8S-induced [Ca2+]i increment from 59.30％ ± 4.85％ to 14.97％ ± 9.05％ (P ＜ 0.01),suggesting a CCK1R-mediated event.Emptying of intracellular calcium stores by thapsigargin (5 μmol/L)prevented CCK-8S (100 nmol/L) from inducing a [Ca2+]i increase.Moreover,pretreatment with xestospongin C (1 μmol/L) could also abolish the CCK-8S-induced effect,indicating that Ca2+ release from InsP3R-operated stores appeared to be a major mechanism responsible for CCK-8S-induced calcium mobilization in ICC.On the other hand,by removing extracellular calcium or blocking the L-type voltage-operated calcium channel with nifedipine,a smaller but significant rise in the [Ca2+]i could be still elicited by CCK-8S.These data suggest that the [Ca2+]i release is not stimulated or activated by the influx of extracellular Ca2+ in ICC,but the influx of extracellular Ca2+ can facilitate the [Ca2+]i increase evoked by CCK-8S.CCK-8S increased the phosphorylation of InsP3R3,which could be prevented by chelerythrine.Pretreatment with lorglumide (5 μmol/L) could significantly reduce the CCK-8S intensified phosphorylation of InsP3R3.In the positive control group,treatment of cells with PMA also resulted in an enhanced phosphorylation of InsP3R3.Pretreatment with various concentrations of PMA (10 nmol/L-10 μmol/L) apparently inhibited the effect of CCK-8S and the effect of 100 nmol/L PMA was most obvious.Likewise,the effect of CCK-8S was augmented by the pretreatment with chelerythrine (10 nmol/L-10 μmol/L) and 100nmol/L chelerythrine exhibited the maximum effect.CONCLUSION:CCK-8S increases [Ca2+]i in ICC via the CCK1 receptor.This effect depends on the release of InsP3R-operated Ca2+ stores,which is negatively regulated by PKC-mediated phosphorylation of InsP3R3.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Establishment of a rat liver transplantation model with prolonged biliary warm ischemia time

    Xin-Hua ZhuJun-Ping PanYa-Fu WuYi-Tao Ding...
    7194-7200页
    查看更多>>摘要：AIM:To investigate the impact of different time points of secondary warm ischemia on bile duct in a rat autologous liver transplantation model with external bile drainage.METHODS:One hundred and thirty-six male inbred SD rats were randomly assigned to one of four groups (Ⅰ-Ⅳ) according to the secondary warm ischemia time of 0,10,20 and 40 min.A rat model of autologous liver transplantation with continuous external biliary drainage under ether anesthesia was established.Ten rats in each group were used to evaluate the one-week survival rate.At 6 h,24 h,3 d and 7 d after reperfusion of the hepatic artery,6 rats were killed in each group to collect the blood sample via the infrahepatic vena cava and the median lobe of liver for assay.Warm ischemia time of liver,cold perfusion time,anhepatic phase,operative duration for biliary external drainage and survival rates in the four groups were analyzed for the establishment of models.RESULTS:No significant difference was shown in warm ischemia time,anhepatic phase and operative duration for biliary external drainage among the four groups.Five of the 40 rats in this study evaluated for the one-week survival rate died,including three deaths of severe pulmonary infection in group Ⅳ.A significant decrease of one-week survival rate in group Ⅳ was noted compared with the other three groups.With the prolongation of the biliary warm ischemia time,the indexes of the liver function assessment were significantly elevated,and biliary epithelial cell apoptosis index also increased.Pathological examinations showed significantly aggravated inflammation in the portal area and bile duct epithelial cell injury with the prolonged secondary warm ischemia time.Microthrombi were found in the micrangium around the biliary tract in some sections from groups Ⅲ and Ⅳ.CONCLUSION:The relationship between secondary warm ischemia time and the bile duct injury degree is time-dependent,and 20 min of secondary warm ischemia time is feasible for the study of bile duct injury.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Electroacupuncture alleviates stress-induced visceral hypersensitivity through an opioid system in rats

    Yuan-Yuan ZhouNatalie J WannerYing XiaoXuan-Zheng Shi...
    7201-7211页
    查看更多>>摘要：AIM:To investigate whether stress-induced visceral hypersensitivity could be alleviated by electroacupuncture (EA) and whether EA effect was mediated by endogenous opiates.METHODS:Six to nine week-old male SpragueDawley rats were used in this study.Visceral hypersensitivity was induced by a 9-d heterotypic intermittent stress (HIS) protocol composed of 3 randomly stressors,which included cold restraint stress at 4 ℃ for 45 min,water avoidance stress for 60 min,and forced swimming stress for 20 min,in adult male rats.The extent of visceral hypersensitivity was quantified by electromyography or by abdominal withdrawal reflex (AWR) scores of colorectal distension at different distention pressures (20 mmHg,40 mmHg,60 mmHg and 80 mmHg).AWR scores either 0,1,2,3 or 4 were obtained by a blinded observer.EA or sham EA was performed at classical acupoint ST-36 (Zu-San-Li) or BL-43 (Gao-Huang) in both hindlimbs of rats for 30 min.Naloxone (NLX) or NLX methiodide (m-NLX) was administered intraperitoneally to HIS rats in some experiments.RESULTS:HIS rats displayed an increased sensitivity to colorectal distention,which started from 6 h (the first measurement),maintained for 24 h,and AWR scores returned to basal levels at 48 h and 7 d after HIS compared to pre-HIS baseline at different distention pressures.The AWR scores before HIS were 0.6 ± 0.2,1.3 ± 0.2,1.9 ± 0.2 and 2.3 ± 0.2 for 20 mmHg,40 mmHg,60 mmHg and 80 mmHg distention pressures,respectively.Six hours after termination of the last stressor,the AWR scores were 2.0 ± 0.1,2.5 ± 0.1,2.8 ± 0.2 and 3.5 ± 0.2 for 20 mmHg,40 mmHg,60 mmHg and 80 mmHg distention pressures,respectively.EA given at classical acupoint ST-36 in both hindlimbs for 30 min significantly attenuated the hypersensitive responses to colorectal distention in HIS rats compared with sham EA treatment [AWRs at 20 mmHg:2.0 ± 0.2 vs 0.7 ± 0.1,P =4.23 711 E-4; AWRs at 40 mmHg:2.6 ± 0.2 vs 1.5 ± 0.2,P =0.00 163; AWRs at 60 mmHg:3.1 ± 0.2 vs 1.9 ± 0.1,P =0.003; AWRs at 80 mmHg:3.6 ± 0.1 vs 2.4 ± 0.2,P =0.0023; electromyographic (EMG) at 20 mmHg:24 ± 4.7 vs13.8 ± 3.5; EMG at 40 mmHg:60.2 ± 6.6 vs 30 ± 4.9,P =0.00 523; EMG at 60 mmHg:83 ± 10 vs 39.8 ± 5.9,P =0.00 029; EMG at 80 mmHg:94.3 ± 10.8 w 49.6 ± 5.9,P =0.00 021].In addition,EA at the acupuncture point BL-43 with same parameters did not alleviate visceral hypersensitivity in HIS rats.EA in healthy rats also did not have any effect on AWR scores to colorectal distention at distention pressures of 20 and 40 mmHg.The EA-mediated analgesic effect was blocked by pretreatment with NLX in HIS rats [AWR scores pretreated with NLX vs normal saline (NS) were 2.0 vs 0.70 ± 0.20,2.80 ± 0.12 Vs 1.50 ± 0.27,3 vs 2.00 ± 0.15 and 3.60 ± 0.18 vs 2.60 ± 0.18 for 20 mmHg,40 mmHg,60 mmHg and 80 mmHg; P =0.0087,0.0104,0.0117 and 0.0188 for 20,40,60 and 80 mmHg,respectively].Furthermore,EA-mediated analgesic effect was completely reversed by administration of m-NLX,a peripherally restricted opioid antagonist (EMG pretreated with m-NLX vs NS were 30.84 ± 4.39 vs 13.33 ± 3.88,74.16 ± 9.04 vs 36.28 ± 8.01,96.45 ± 11.80 vs 50.19 ± 8.28,and 111.59 ± 13.79 vs 56.42 ± 8.43 for 20 mmHg,40 mmHg,60 mmHg and 80 mmHg; P =0.05 026,0.00 034,0.00 005,0.000 007 for 20 mmHg,40 mmHg,60 mmHg and 80 mmHg,respectively).CONCLUSION:EA given at classical acupoint ST-36 alleviates stress-induced visceral pain,which is most likely mediated by opioid pathways in the periphery.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普