首页|B1a lymphocytes in the rectal mucosa of ulcerative colitis patients

B1a lymphocytes in the rectal mucosa of ulcerative colitis patients

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AIM: To assess B1a cell expression in the rectal mucosa of ulcerative colitis (UC) patients in comparison with healthy controls. METHODS: Rectal mucosa biopsies were collected from 15 UC patients and 17 healthy controls. CD5+ B cells were analysed by three colour flow cytometry from rectal mucosal samples after mechanical disaggregation by Medimachine?. Immunohistochemical analysis of B and T lymphocytes was also performed. Correlations between, on the one hand, rectal B1a cell concentrations and, on the other, erythrocyte sedimentation rate and C-reactive protein levels and clinical, endoscopic and histological disease activity indices were evaluated.RESULTS: Rectal B-lymphocyte (CD19+/CD45+) rate and concentration were higher in UC patients compared with those in healthy controls (47.85% ± 3.12% vs 26.10% ± 3.40%, P = 0.001 and 501 ± 91 cells/ mm2 vs 117 ± 18 cells/mm2, P < 0.001); Rectal B1a cell density (CD5+CD19+) was higher in UC patients than in healthy controls (85 ± 15 cells/mm2 vs 31 ± 6.7 cells/mm2, P = 0.009). Rectal B1a cell (CD5/CD19+) rate correlated inversely with endoscopic classification (Rs = -0.637, P < 0.05). CONCLUSION: B1a lymphocytes seem to be involved in the pathogenesis of UC, however, the role they play in its early phases and in disease activity, have yet to be defined.

B1 cellCD5Flow cytometryRectumUlcerative colitis

Lino Polese、Riccardo Boetto、Giuseppe De Franchis、Imerio Angriman、Andrea Porzionato、Lorenzo Norberto、Giacomo Carlo Sturniolo、Veronica Macchi、Raffaele De Caro、Stefano Merigliano

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Department of Surgical and Gastroenterological Sciences, University of Padova, Via Giustiniani 2, 35128 Padova , Italy

Department of Laboratory Medicine, Padova University Hospital, Via Giustiniani 2, 35128 Padova, Italy

Department of Human Anatomy and Physiology, University of Padova, Via Gabelli 65, 35128 Padova, Italy

2012

10.3748/wjg.v18.i2.144

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(2)
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