首页|Human epidermal growth factor receptor-2 gene amplification in gastric cancer using tissue microarray technology

Human epidermal growth factor receptor-2 gene amplification in gastric cancer using tissue microarray technology

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AIM: To assess human epidermal growth factor receptor- 2 (HER2)-status in gastric cancer and matched lymph node metastases by immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH). METHODS: 120 cases of primary gastric carcinomas and 45 matched lymph node metastases from patients with full clinicopathological features were mounted onto multiple-punch and single-punch tissue microarrays, respectively, and examined for HER2 overexpression and gene amplification by IHC and CISH. RESULTS: Twenty-four tumors (20%) expressed HER2 immunohistochemically. An IHC score of ≥ 2+ was observed in 20 tumors (16.6%). HER2 amplification was detected by CISH in 19 tumors (15.8%) and in their matched lymph node metastases. A high concordance rate was found between HER2 positivity (as detected by IHC) and HER2 gene amplification (as detected by CISH), since 19 of the 20 IHC positive cases were amplified (95%). All amplified cases had 2+ or 3+ IHC results. Amplification was associated with intestinal phenotype (P < 0.05). No association with grading, staging or survival was found. CONCLUSION: In gastric cancer, HER2 amplification is the main mechanism for HER2 protein overexpression and is preserved in lymph node metastases.

Human epidermal growth factor receptor-2ImmunohistochemistryChromogenic in situ hybridizationGastric cancer

Dimitrios Tsapralis、Ioannis Panayiotides、George Peros、Theodore Liakakos、Eva Karamitopoulou

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3rd Department of Surgery, Attikon University Hospital, University of Athens, Rimini 1, 12464 Athens, Greece

2nd Department of Pathology, University of Athens, Rimini 1, 12464 Athens, Greece

4th Department of Surgery, Attikon University Hospital, University of Athens, Rimini 1, 12464 Athens, Greece Eva Karamitopoulou, Department of Pathology, Aretaieion University Hospital, University of Athens, Vass. Sophias 76, 11528 Athens, Greece

Department of Pathology, Aretaieion University Hospital, University of Athens, Vass. Sophias 76, 11528 Athens, Greece

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University of Athens

2012

10.3748/wjg.v18.i2.150

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(2)
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