首页|Stress-induced visceral analgesia assessed non-invasively in rats is enhanced by prebiotic diet

Stress-induced visceral analgesia assessed non-invasively in rats is enhanced by prebiotic diet

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AIM: To investigate the influence of repeated water avoidance stress (rWAS) on the visceromotor response (VMR) to colorectal distension (CRD) and the modulation of the response by a prebiotic diet in rats using a novel surgery-free method of solid-state manometry.METHODS: Male Wistar rats fed a standard diet with or without 4% enzyme-treated rice fiber (ERF) for 5 wk were subjected to rWAS (1 h daily x 10 d) or no stress. The VMR to graded phasic CRD was assessed by intraluminal colonic pressure recording on days 0 (baseline), 1 and 10 (45 min) and 11 (24 h) after rWAS and expressed as percentage change from baseline. Cecal content of short chain fatty acids and distal colonic histology were assessed on day 11.RESULTS: WAS on day 1 reduced the VMR to CRD at 40 and 60 mmHg similarly by 28.9% ± 6.6% in both diet groups. On day 10, rWAS-induced reduction of VMR occurred only at 40 mmHg in the standard diet group (36.2% ± 17.8%) while in the ERF group VMR was lowered at 20, 40 and 60 mmHg by 64.9% ± 20.9%, 49.3% ± 11.6% and 38.9% ± 7.3% respectively. The visceral analgesia was still observed on day 11 in ERF- but not in standard diet-fed rats. By contrast the non-stressed groups (standard or ERF diet) exhibited no changes in VMR to CRD. In standard diet-fed rats, rWAS induced mild colonic histological changes that were absent in ERF-fed rats exposed to stress compared to non-stressed rats. The reduction of cecal content of isobutyrate and total butyrate, but not butyrate alone, was correlated with lower visceral pain response. Additionally, ERF diet increased rWAS-induced defecation by 26% and 75% during the first 0-15 min and last 15-60 min, respectively, compared to standard diet, and reduced rats' body weight gain by 1.3 fold independently of their stress status. CONCLUSION: These data provide the first evidence of psychological stress-related visceral analgesia in rats that was enhanced by chronic intake of ERF prebiotic.

Stress-related visceral analgesiaWater avoidance stressColorectal distensionEnzyme-treated rice fiber prebioticShort chain fatty acidsDefecationRatSolid-state manometry

Muriel Larauche、Agata Mulak、Pu-Qing Yuan、Osamu Kanauchi、Yvette Taché

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CURE: Digestive Diseases Research Center and Oppenheimer Family Center for Neurobiology of Stress, Department of Medicine, Digestive Diseases Division at the University of California Los Angeles, VA Greater Los Angeles Healthcare System, Los Angeles, CA 90073, United States

Department of Gastroenterology and Hepatology, Wroclaw Medical University, Borowska 213, 50-556 Wroclaw, Poland

Shiga University of Medical Science, Shiga and Central Laboratories for Frontier Technology, Kirin Holdings Co. Ltd., 1-13-5, Fukuura, Kanazawa-ku, Yokohama, 236-0004, Japan

Supported by Central Labs for Frontier Technology Kirin Holdings Co.,Ltd Japan and the National Institute of Health grantsSupported by Central Labs for Frontier Technology Kirin Holdings Co.,Ltd Japan and the National Institute of Health grantsSupported by Central Labs for Frontier Technology Kirin Holdings Co.,Ltd Japan and the National Institute of Health grantsSupported by Central Labs for Frontier Technology Kirin Holdings Co.,Ltd Japan and the National Institute of Health grantsSupported by Central Labs for Frontier Technology Kirin Holdings Co.,Ltd Japan and the National Institute of Health grantsVeterans Administration Research Career Scientist Award

P50 DK-64539 to Taché YLarauche MCenter Grant DK-41301 Animal Coreto Yvette TachéR01 DK-

2012

10.3748/wjg.v18.i3.225

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(3)
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