首页|Effects and mechanisms of store-operated calcium channel blockade on hepatic ischemia-reperfusion injury in rats

Effects and mechanisms of store-operated calcium channel blockade on hepatic ischemia-reperfusion injury in rats

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AIM: To further investigate the important role of storeoperated calcium channels (SOCs) in rat hepatocytes and to explore the effects of SOC blockers on hepatic ischemia-reperfusion injury (HIRI). METHODS: Using freshly isolated hepatocytes from a rat model of HIRI (and controls), we measured cytosolic free Ca2+ concentration (by calcium imaging), net Ca2+ fluxes (by a non-invasive micro-test technique), the SOC current (ISOC; by whole-cell patch-clamp recording), and taurocholate secretion [by high-performance liquid chromatography and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays]. RESULTS: Ca2+ oscillations and net Ca2+ fluxes mediated by Ca2+ entry via SOCs were observed in rat hepatocytes. ISOC was significantly higher in HIRI groups than in controls (57.0 ± 7.5 pA vs 31.6 ± 2.7 pA, P <0.05) and was inhibited by La3+. Taurocholate secretion by hepatocytes into culture supernatant was distinctly lower in HIRI hepatocytes than in controls, an effect reversed by SOC blockers. CONCLUSION: SOCs are pivotal in HIRI. SOC blockers protected against HIRI and assisted the recovery of secretory function in hepatocytes. Thus, they are likely to become a novel class of effective drugs for prevention or therapy of HIRI patients in the future.

HepatocyteHepatic ischemia-reperfusion injuryStore-operated calcium channelNon-invasive micro-test technique

Li-Jie Pan、Zi-Chao Zhang、Zhen-Ya Zhang、Wen-Jun Wang、Yue Xu、Zong-Ming Zhang

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Department of General Surgery, Digestive Medical Center, The First Affiliated Hospital, School of Medicine, Tsinghua University, Beijing 100016, China

Xuyue Beijing Science and Technology Co., Ltd., Haidian District, Beijing 100080, China

国家自然科学基金国家自然科学基金Tsinghua-Yue-Yuen Medical Science FoundationTsinghua-Yue-Yuen Medical Science Foundation

30670744810719962024000053120240000547

2012

10.3748/wjg.v18.i4.356

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(4)
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