首页|A Macaca mulatta model of fulminant hepatic failure

A Macaca mulatta model of fulminant hepatic failure

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AIM: To establish an appropriate primate model of fulminant hepatic failure (FHF). METHODS: We have, for the first time, established a large animal model of FHF in Macaca mulatta by intraperitoneal infusion of amatoxin and endotoxin. Clinical features, biochemical indexes, histopathology and iconography were examined to dynamically investigate the progress and outcome of the animal model. RESULTS: Our results showed that the enzymes and serum bilirubin were markedly increased and the enzyme-bilirubin segregation emerged 36 h after toxin administration. Coagulation activity was significantly decreased. Gradually deteriorated parenchymal abnormality was detected by magnetic resonance imaging (MRI) and ultrasonography at 48 h. The liver biopsy showed marked hepatocyte steatosis and massive parenchymal necrosis at 36 h and 49 h, respectively. The autopsy showed typical yellow atrophy of the liver. Hepatic encephalopathy of the models was also confirmed by hepatic coma, MRI and pathological changes of cerebral edema. The lethal effects of the extrahepatic organ dysfunction were ruled out by their biochemical indices, imaging and histopathology. CONCLUSION: We have established an appropriate large primate model of FHF, which is closely similar to clinic cases, and can be used for investigation of the mechanism of FHF and for evaluation of potential medical therapies.

Fulminant hepatic failureMacaca mulattaBiochemistryImagingPathology

Ping Zhou、Hong Bu、Jie Xia、Gang Guo、Li Li、Yu-Jun Shi、Zi-Xing Huang、Qiang Lu、Hong-Xia Li

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Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China

Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Provinc

Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China

Department of Radiology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China

Department of Ultrasound, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China

The National Chengdu Center for Safety Evaluation of Drugs, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China

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国家重点基础研究发展规划(973计划)国家自然科学基金国家自然科学基金

2009CB5224013087098330971118

2012

10.3748/wjg.v18.i5.435

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(5)
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