首页|Safety assessment of Bifidobacterium longum J DM301 based on complete genome sequences

Safety assessment of Bifidobacterium longum J DM301 based on complete genome sequences

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AIM: To assess the safety of Bifidobacterium longum (B.longum) JDM301 based on complete genome sequences. METHODS: The complete genome sequences of JDM301 were determined using the GS 20 system. Putative virulence factors, putative antibiotic resistance genes and genes encoding enzymes responsible for harmful metabolites were identified by blast with virulence factors database, antibiotic resistance genes database and genes associated with harmful metabolites in previous reports. Minimum inhibitory concentration of 16 common antimicrobial agents was evaluated by E-test. RESULTS: JDM301 was shown to contain 36 genes associated with antibiotic resistance, 5 enzymes related to harmful metabolites and 162 nonspecific virulence factors mainly associated with transcriptional regulation, adhesion, sugar and amino acid transport. B. longum JDM301 was intrinsically resistant tocipro ciprofloxacin,amikacin, gentamicin and streptomycin and susceptible to vancomycin, amoxicillin, cephalothin, chloramphenicol, erythromycin, ampicillin, cefotaxime, rifampicin, imipenemandtrimethoprim and trimethoprim-sulphamethoxazol. JDM301.JDM301 was moderately resistant to bacitracin, while an earlier study showed that bifidobacteria were susceptible to this antibiotic. A tetracycline resistance gene with the risk of transfer was found in JDM301, which needs to be experimentally validated. CONCLUSION: The safety assessment of JDM301 using information derived from complete bacterial genome will contribute to a wider and deeper insight into the safety of probiotic bacteria.

Bifidobacterium longumSafety assessmentGenomeAntibiotic resistanceHarmful metaboliteVirulence factor

Yan-Xia Wei、Zhuo-Yang Zhang、Chang Liu、Xiao-Kui Guo、Pradeep K Malakar

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Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Institute of Food Research, Norwich Research Park, NR4 7UA, Norwich, United Kingdom

National Key Program for Infectious Diseases of ChinaNational Key Program for Infectious Diseases of ChinaProgram of Shanghai Subject Chief ScientistProgram of Shanghai Research and DevelopmentChina Partnering Award from the Biotechnology and Biological Sciences Research Council,United Kingdom

2008ZX100042009ZX1000409XD140270010JC1408200

2012

10.3748/wjg.v18.i5.479

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(5)
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