首页|Study on RIZ1 gene promoter methylation status in human esophageal squamous cell carcinoma

Study on RIZ1 gene promoter methylation status in human esophageal squamous cell carcinoma

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AIM: To investigate the promoter region methylation status of retinoblastoma protein-interacting zinc finger gene 1 (RIZ1) in the human esophageal squamous cell carcinoma (ESCC) cell lines and tissues and verify the relationship between methylation of RIZ1 and oncogen-esis, tumor progression and metastasis etc of ESCC.METHODS: Methylation-specific polymerase chain reaction (MSP) was used to investigate the promoter region methylation status of RIZ1 in 6 ESCC cell lines. One cell line where RIZ1 promoter region methylation was detected was selected for the next study, where the cell line was treated with 5-aza-CdR. Real-time polymerase chain reaction was used to investigate its influence on the transcription of RIZ1. Experiments using frozen pathological specimens from 47 ESCC patients were performed using the same MSP methodology.RESULTS: Promoter methylation of RIZ1 gene was detected in TE13, CaEs17 and EC109 cell lines and the cell line TE13 was chosen for further study. The expression of RIZ1 mRNA in TE-13 was up-regulated after treatment with 5-aza-CdR. The rate of methylation in carcinomas tissues was significantly higher than those in matched neighboring normal and distal ending normal tissue, and the deviation of data was statistically significant (x2 = 24.136, P < 0.01). Analysis of the gender, age familial history, tumour deviation, tumour saturation, lymph gland displacement and clinical staging of 47 samples from ESCC patients showed that the fluctuation of data was not statistically significant.CONCLUSION: Promoter methylation may play an important role in the epigenetic silencing of RIZ1 gene expression in human ESCC. RIZ1 is considered to be a potential tumor suppressor gene and may be a biological parameter for testing early stage human ESCC.

Retinoblastoma protein-interacting zinc finger gene 1Tumor suppressor genesEsophageal squamous cell carcinomaPromoter methylationMethylation-specific polymerase chain reaction

Shang-Wen Dong、Peng Zhang、Yi-Mei Liu、Yuan-Tao Cui、Shuo Wang、Shao-Jie Liang、Zhun He、Pei Sun、Yuan-Guo Wang

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Department of Cardiothoracic Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China

Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, China

Supported by Grant-in-aid from Specialized Research Fund for the Doctoral Program of Higher EducationGrant-in-aid from Natural Science Foundation of Tianjin

2009120211000910JCYBJC11300

2012

10.3748/wjg.v18.i6.576

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(6)
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