首页|Expression of fibroblast activation protein in human pancreatic adenocarcinoma and its clinicopathological significance
Expression of fibroblast activation protein in human pancreatic adenocarcinoma and its clinicopathological significance
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NETL
NSTL
万方数据
维普
AIM:To examine fibroblast activation protein (FAP)expression in pancreatic ductal adenocarcinoma (PDAC)and to analyze its relationship with the clinicopathology of PDAC.METHODS:FAP expression was examined in 134 PDAC specimens by immunohistochemistry,and in four pancreatic cancer cell lines (SW1990,Miapaca-2,AsPC-1 and BxPC-3) by Western blotting assay.We also analyzed the association between FAP expression in PDAC cells and the clinicopathology of PDAC patients.RESULTS:The results showed that the FAP was expressed in both stromal fibroblast cells (98/134,73.1％) and carcinoma cells (102/134,76.1％).All 4 pancreatic cancer cell lines expressed FAP protein at different levels.Protein bands corresponding to the proteolytically active 170-kDa seprase dimer and its 88-kDa seprase subunit were identified.Higher FAP expression in carcinoma cells was associated with tumor size (P ＜ 0.001),fibrotic focus (P =0.003),perineural invasion (P =0.009) and worse clinical outcome (P =0.0085).CONCLUSION:FAP is highly expressed in carcinoma cells and fibroblasts in PDAC tissues,and its expression is associated with desmoplasia and worse prognosis.
NETL
NSTL
万方数据
维普
