首页|High-fat-induced intestinal permeability dysfunction associated with altered fecal bile acids
High-fat-induced intestinal permeability dysfunction associated with altered fecal bile acids
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维普
AIM:To investigate whether high-fat-feeding is associated with increased intestinal permeability via alterations in bile acid metabolism.METHODS:Male C57B1/6J mice were fed on a high-fat (n =26) or low-fat diet (n =24) for 15 wk.Intestinal permeability was measured from duodenum,jejunum,ileum and colon in an Ussing chamber system using 4 kDa FITC-labeled dextran as an indicator.Fecal bile acids were analyzed with gas chromatography.Segments of jejunum and colon were analyzed for the expression of farnesoid X receptor (FXR) and tumor necrosis factor (TNF).RESULTS:Intestinal permeability was significantly increased by high-fat feeding in jejunum (median 0.334 for control vs 0.393 for high-fat,P =0.03) and colon (0.335 for control vs 0.433 for high-fat,P =0.01),but not in duodenum or ileum.The concentration of nearly all identified bile acids was significantly increased by high-fat feeding (P ＜ 0.001).The proportion of ursodeoxycholic acid (UDCA) in all bile acids was decreased (1.4％ ± 0.1％ in high-fat vs 2.8％ ± 0.3％ in controls,P ＜ 0.01) and correlated inversely with intestinal permeability (r =-0.72,P =0.01).High-fat feeding also increased jejunal FXR expression,as well as TNF expression along the intestine,especially in the colon.CONCLUSION:High-fat-feeding increased intestinal permeability,perhaps by a mechanism related to bile acid metabolism,namely a decreased proportion of fecal UDCA and increased FXR expression.
Bile acidsBile saltsDiet-induced obesityFarnesoid X-activated receptorIntestinal permeabilityUrsodeoxycholic acid
Lotta K Stenman、Reetta Holma、Riitta Korpela
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Institute of Biomedicine, Pharmacology, University of Helsinki, Helsinki 00280, Finland
NETL
NSTL
万方数据
维普
