首页|Genetic characteristics and pathogenicity of human hepatitis E virus in Nanjing, China
Genetic characteristics and pathogenicity of human hepatitis E virus in Nanjing, China
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AIM:To investigate the genetic characteristics and pathogenicity of hepatitis E virus (HEV) and assess the potential risk factors for sporadic hepatitis E.METHODS:Sixty-two serum samples from the patients with acute hepatitis E were collected,including 23 cases coinfected with hepatitis B virus.Anti-HEV detection and partial HEV RNA amplification were performed by enzyme immunoassays and reverse transcriptionnested polymerase chain reaction (RT-nPCR) method,respectively,and PCR products were sequenced.The isolated human HEV sequences were analyzed phylogenetically.RESULTS:The positive rate of serum HEV RNA were 21.0％ (13/62),including 5 cases of liver failure.All the 13 isolates shared a 82.1％-98.0％ nucleotide homology with each other and had identities of 74.7％-81.0％,75.3％-78.6％,75.3％-80.0％ and 82.1％-96.1％ with the corresponding regions of HEV genotypes 1-4,respectively.The human HEV strain GS-NJ-12 shared a 100％ nucleotide identity with the swine HEV strain swIM6-43 isolated from Inner Mongolia,China.CONCLUSION:Swine may be a principal risk factor for occurrence of sporadic hepatitis E in eastern China,and genotype 4 HEV can induce acute liver failure.
GenotypeHepatitis E virusLiver failureZoonotic transmissionPathogenicity
Jia-Bao Geng、Mao-Rong Wang、Ling Wang、Jie Wang、Zhi-Guo Yang、Yan Cheng、Fei Qiao、Min Wang
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Institute of Liver Disease,Nanjing Bayi Hospital, Nanjing 210002, Jiangsu Province, China
Department of Microbiology, Peking University Health Science Center, Beijing 100091, China
863 National High Technology Research and Development Program of China国家自然科学基金
NETL
NSTL
万方数据
维普
