首页|Serological and molecular study of hepatitis E virus among illegal blood donors

Serological and molecular study of hepatitis E virus among illegal blood donors

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AIM:To investigate the seroprevalence and molecular characteristics of hepatitis E virus (HEV) in the illegal blood donors (IBDs) of central China in the early 1990s.METHODS:A total of 546 blood samples were collected from the IBDs in Maanshan city,a questionnaire was completed by each subject,detailing the age,sex,and periods of blood or plasma donation.Anhui Province and tested for the anti-HEV antibodies.The seropositive samples were subjected to nested reverse transcriptionpolymerase chain reaction and sequencing to analyze HEV partial genome.RESULTS:The prevalence of IgG and IgM HEV antibody in IBDs was 22.7％ and 1.8％,and genotype 4 was the dominant circulating HEV type in IBDs.The prevalence of anti-HEV IgG was significantly related to sex (OR =4.905,P =0.004) and increased with age (OR =2.78,P =0.022),which ranged from 13.0％ in those ＜ 40 years old to 30.6％ among older persons aged ＞ 60 years.Moreover,frequency of blood donation was significantly associated with HEV seropositivity (OR =2.06,P =0.006).HEV partial sequences of ORF2 and obtained 3 sequences in serum samples of 10 IBDs which developed HEV specific IgM CONCLUSION:This study helps define one of the possible routes of transmission of sporadic HEV infection and provides guidance to screen HEV in the blood donors so as to guarantee safe blood banks in China.

MolecularSero-epidemiologyHepatitis EHepatitis E virusCommercial blood donors

Xian-Feng Cheng、Yu-Feng Wen、Ming Zhu、Sheng-Wei Zhan、Jin-Xiu Zheng、Chen Dong、Ke-Xia Xiang、Xiao-Bing Xia、Gang Wang、Ling-Fei Han

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Ma Anshan Center for Disease Control and Prevention, Maanshan 243000,Anhui Province, China

Department of Preventive Medicine, Wannan Medical College, Wuhu 241320, Anhui Province,China

Department of Microbiology and Immunology,School of Medicine, Southeast University, Nanjing 210096, Jiangsu Province, China

Department of Clinical Laboratory, the Forth People's Hospital of Maanshan, Maanshan 243000, Anhui Province,China

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Natural Science Foundation of Maanshan,China (2008-40) (To Zhan SW and Zheng JX)

2012

10.3748/wjg.v18.i9.986

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(9)
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